Human Hsp 70 ELISA Kit (DEIA1972)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum
Species Reactivity
Human
Intended Use
The Human Hsp 70 Antibody ELISA(enzyme-linked immunosorbent assay) Kit provides a method to detect and quantitate antibodies to human Hsp70 in human serum samples.
This assay allows for reproducible, accurate and precise determination of IgG, IgA and IgM antibodies to human Hsp70.
Contents of Kit
1. Immunoassay Plate: 12 x 8 removable strips and frame. Pre-coated plate with recombinant human Hsp70 antigen
2. Standard: Human serum containing antihuman Hsp70 IgG, IgA, IgM antibodies
3. Sample Diluent: Buffer to dilute standards and samples
4. 20X Wash Buffer: Concentrated solution of buffer and surfactant
5. Anti-Human GAM-HRP Conjugate: Horseradish Peroxidase conjugated polyclonal antibody specific for human IgA, IgG, IgM antibodies
6. TMB Substrate Solution: Stabilized tetramethylbenzidine substrate
7. Stop Solution: Acid solution to stop color reaction
Storage
Store the unopened kit at 4°C upon receipt and when it is not in use. For more detailed information, please download the following document on our website.
Sensitivity
6.79 ng/mL

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References


Impact of heat stress on expression kinetics of HSP27 in cardiac cells of goats

BIOLOGICAL RHYTHM RESEARCH

Authors: Parida, S.; Mishra, S. R.; Mishra, C.; Dalai, N.; Mohapatra, S.; Mahapatra, A. P. K.; Kundu, A. K.

The present study was aimed to investigate the effect of heat stress on the expression kinetics of heat shock protein 27 (HSP27) mRNA in cultured cardiac cells of goats. The heart tissues (n= 6) from different goats were used for the culture study. The cardiac cells obtained from different heart tissues were cultured in 24-well culture plates and incubated in a humidified CO2(5%) incubator at 37 degrees C. The cardiac cells were allowed to become 75-80% confluent after 72 h of incubation. Thereafter, the cardiac cells were subjected to heat exposure at 42 degrees C (heat exposed) for 0, 20, 60 and 100 min. The cardiac cells exposed to heat stress at 42 degrees C for 0 min was taken as control. The relative abundance of HSP27 mRNA did not change at 20 min of heat exposure and increased (P< 0.05) at 60 and 100 min of heat exposure as compared to control. However, the highest (P< 0.05) relative abundance of HSP27 mRNA was noticed at 100 min of heat exposure. The present findings indicate that HSP27 could possibly promote thermo-protection to cardiac cell from the detrimental effect of heat stress.

Comprehensive analysis of differentially expressed proteins in the male and femaleBombyx morilarval instars exposed to thermal stress

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

Authors: Punyavathi; Manjunatha, H. B.

Gender sensitivity to ambient heat, despite well known in insect species, how it manifests during young and late larval instars ofBombyx moriis still unclear. To uncover this cryptic feature, different instars male and female larvae were subjected to varied thermal stress separately and sex-stage specific expression of proteins was investigated. Interestingly, heat shock proteins (HSPs) 90 and 70 were expressed differently in all the instars and also between male and female larvae as confirmed by immunoblot assay. Besides up- and downregulation of few HSPs and other normal proteins, discreet expression of protein was noticed in the two-dimensional gel electrophoresis of male than female larvae which were identified as HSP70 by mass spectrometry. Furthermore, quantitative polymerase chain reaction results show 3.98- and 4.86-fold higher levels ofBmhsp70andBmhsp90transcripts in male and female larvae, respectively, as a response to 40 degrees C heat shock (HS) treatment. Conversely, in spite of the massive production of HSPs due to HS at 45 degrees C, all the larvae were found dead, which is a strong proof of concept for autophagy. Comparatively, female larvae HS at 40 degrees C succeed to spin cocoons with increased weight and silk contents than non-HS larvae. Comprehensively, in the present study, we have noticed a strong correlation for the first time thatBmhsp90andBmhsp70genes expressions due to thermal stress are not only sex specific but also explicit preferential and coordinated action on survivability and biosynthetic potential of the silkworm,B. morilarvae during different instars.

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