Biochemical Toxicity and Potential Detoxification Mechanisms in EarthwormsEisenia fetidaExposed to Sulfamethazine and Copper
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Authors: Rong, Hong; Wang, Chengrun; Liu, Haitao; Zhang, Min; Yuan, Yueting; Pu, Yanjie; Huang, Jin; Yu, Jinyu
Abstract
The present study investigated the biochemical toxicity and potential detoxification mechanisms in earthwormsEisenia fetidaexposed to sulfamethazine (SMZ) (7.5, 15 and 30 mg kg(-1)) either alone or in combination with Copper (Cu) (100 mg kg(-1)) in soil. The results showed that increasing concentrations of SMZ in soil activated superoxide dismutase, catalase and glutathione peroxidase isozymes, suggesting reactive oxygen species (ROS) burst in earthworms. Treatment with SMZ and Cu separately or in combination caused protein oxidation and damage, elevating the synthesis of ubiquitin, the 20S proteasome, cytochrome P450 (CYP450), and heat shock protein 70 (HSP70). Such treatments also induced the activities of proteases, endoproteinase (EP) and glutathione S-transferases (GSTs). The results suggested that the ubiquitin-20S proteasome, proteases, EP and HSP70 were involved in degradation or remediation of oxidatively damaged proteins. Elevated levels of CYP450 and GSTs also participated in the detoxification of the earthworms.
Hsp70 Gene Polymorphisms Are Associated With Disease Susceptibility and HRQOL Improvement in Chinese Han Population With Systemic Lupus Erythematosus
JCR-JOURNAL OF CLINICAL RHEUMATOLOGY
Authors: Xie, Qiao-Mei; Lou, Qiu-Yue; Huang, Shun-Wei; Hu, Hua-Qing; Li, Su-Su; Zhang, Man; Sun, Xiu-Xiu; Xu, Jian-Hua; Jiang, Shan-Qun; Liu, Sheng-Xiu; Xu, Sheng-Qian; Cai, Jing; Liu, Shuang; Pan, Fa-Ming; Tao, Jin-Hui; Qian, Long; Wang, Chun-Huai; Liang, Chun-Mei; Huang, Hai-Liang; Pan, Hai-Feng; Su, Hong; Zou, Yan-Feng
Abstract
Objectives The aim of this study is to investigate whether heat shock protein 70 (Hsp70) gene polymorphisms are implicated in systemic lupus erythematous (SLE) susceptibility, the efficacy of glucocorticoids (GCs) treatment, and improvement of health-related quality of life. Methods A total of 499 SLE patients and 499 controls were included in a case-control study, and 468 SLE patients treated with GCs for 12 weeks were involved in a follow-up study. Patients who completed the 12-week follow-up were divided into GCs-sensitive and GCs-insensitive group by using the SLE disease activity index. The SF-36 was used to evaluate the health-related quality of life of SLE patients, and genotyping was performed by improved multiplex ligation detection reaction. Results rs2075800 was associated with SLE susceptibility (adjusted odds ratio [ORadj], 1.437; 95% confidence interval [CI], 1.113-1.855;P-adj= 0.005;P-BH= 0.020 by dominant model; ORadj, 1.602; 95% CI, 1.072-2.395;P-adj= 0.022;P-BH= 0.029 by TT vs CC model; ORadj= 1.396; 95% CI = 1.067-1.826;P-adj= 0.015;P-BH= 0.029 by TC vs CC model). In the follow-up study, rs2075799 was associated with the improvement in mental health (p= 0.004,P-BH= 0.044), but we failed to find any association between the efficacy of GCs and Hsp70 gene polymorphisms. Conclusions Hsp70 gene polymorphisms may be associated with susceptibility to SLE and improvement of mental health in Chinese Han population.
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