Human Soluble HSP70 ELISA Kit (DEIA-XY2241)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell Lysates, serum, EDTA plasma
Species Reactivity
Human, Mouse, Rat
Intended Use
For the quantitative determination of human shsp70 concentrations in cell lysates, serum and EDTA plasma.
Contents of Kit
1. Soluble HSP70 Microplate: 1 plate
2. Soluble HSP70 Standard: 2 vials
3. Detection Antibody Concentrate: 1 vial
4. Positive Control: 1 vial
5. Streptavidin-HRP Conjugate: 1 vial
6. Dilution Buffer: 1 bottle
7. Wash Buffer: 1 bottle
8. TMB Substrate Solution: 1 bottle
9. Stop Solution: 1 bottle
10. Plate Sealer: 1 piece
11. Plastic Pouch: 1 piece
Storage
2-8°C
Precision
Intra-assay Precision: 4-6%; Inter-assay Precision: 8-10%
Detection Range
156.25-20000 pg/Ml
Sensitivity
50 pg/mL
Standard Curve

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References


Chamazulene-Rich Artemisia arborescens Essential Oils Affect the Cell Growth of Human Melanoma Cells

PLANTS-BASEL

Authors: Russo, Alessandra; Bruno, Maurizio; Avola, Rosanna; Cardile, Venera; Rigano, Daniela

Artemisia arborescens is an aromatic shrub whose essential oils are considered a potential source of molecules with industrial and pharmaceutical interest. The chemical profile ofA. arborescens essential oils (EOs) was shown to be quite variable and various chemotypes have been identified. In this study, we compared the EOs composition of A. arborescens leaves and flowers collected from four different locations in Sicily. The EOs were assayed for their antiproliferative activity against A375 human malignant melanoma cells, also testing cell viability and cell membrane integrity. The evaluation of DNA fragmentation and caspase-3 activity assay was employed for the detection of apoptosis. The expression of Bcl-2, Bax, cleaved caspase-9, PTEN (Phosphatase and tensin homolog), Hsp70 (Heat Shock Protein 70 kilodaltons) and SOD (superoxide dismutase) proteins was evaluated by Western blot analysis. The levels of ROS and GSH were also analyzed. Results show that EOs presented significant differences in their composition, yield, and cytotoxic activity depending on the collection site. The chamazulene/camphor-rich EOs from plants collected in Acqua Calda (Lipari) resulted particularly active on melanoma cancer cells (IC50 values of 6.7 and 4.5 mu g/mL), being able to trigger apoptotic death probably interfering with endogenous defense mechanisms. These oils may be considered as a natural resource of chamazulene, containing this compound up to 63%.

Opposing effects of an F-box protein and the HSP90 chaperone network on microtubule stability and neurite growth in Caenorhabditis elegans

DEVELOPMENT

Authors: Zheng, Chaogu; Atlas, Emily; Lee, Ho Ming Terence; Jao, Susan Laura Javier; Nguyen, Ken C. Q.; Hall, David H.; Chalfie, Martin

Molecular chaperones often work collaboratively with the ubiquitylation-proteasome system (UPS) to facilitate the degradation of misfolded proteins, which typically safeguards cellular differentiation and protects cells from stress. In this study, however, we report that the Hsp70/Hsp90 chaperone machinery and an F-box protein, MEC-15, have opposing effects on neuronal differentiation, and that the chaperones negatively regulate neuronal morphogenesis and functions. Using the touch receptor neurons (TRNs) of Caenothabditis elegans, we find that mec-15(-) mutants display defects in microtubule formation, neurite growth, synaptic development and neuronal functions, and that these defects can be rescued by the loss of Hsp70/Hsp90 chaperones and co-chaperones. MEC-15 probably functions in a Skp-, Cullin- and F-box-containing complex to degrade DLK-1, which is an Hsp90 client protein stabilized by the chaperones. The abundance of DLK-1, and likely other Hsp90 substrates, is fine-tuned by the antagonism between MEC-15 and the chaperones; this antagonism regulates TRN development, as well as synaptic functions of GABAergic motor neurons. Therefore, a balance between the UPS and the chaperones tightly controls neuronal differentiation.

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