Anti-SMC3 Polyclonal Antibody (CABT-L2125) Knockout Validated

Rabbit Anti-Human SMC3 (Ser994~Glu1181) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Recombinant fragment corresponding to human SMC3 (Ser994~Glu1181)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.5-2 ug/ml; 1:250-1000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type Jurkat cell lysate. Lane 2: SMC3 knockout Jurkat cell lysate. Predicted MW: 142kd, Observed MW: 142kd
Figure. Western Blot. Sample. Lane1: Human HepG2 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.

Target


Alternative Names
BMH; BAM; CSPG6; HCAP; SMC3L1; Chondroitin Sulfate Proteoglycan 6
Entrez Gene ID
UniProt ID

Product Background


Pathway
Cell Cycle; Cell Cycle, Mitotic; Cell cycle; Cohesin Loading onto Chromatin; Establishment of Sister Chromatid Cohesion; M Phase; Meiosis; Meiotic synapsis;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Krasikova, A; Barbero, JL; et al. Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes. CHROMOSOME RESEARCH 13:675-685(2005).
Lee, J; Iwai, T; et al. Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis. JOURNAL OF CELL SCIENCE 116:2781-2790(2003).

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