Vitamin B12 [KLH]

Name: Vitamin B12 [KLH]
SKU: DAG3039
Rating: 5 (1 reviews)

Vitamin B12-KLH, synthetic

COA

SDS

Datasheet

Summary

Target

VB12

Nature

Synthetic

Tag/Conjugate

KLH

Alternative Names

Vitamin B12; Cyanocobalamin; Cobalamin

Dilution

20-30

Format

Liquid

Concentration

1 mg/ml

Buffer

10mM PBS, pH7.4

Preservative

None

Storage

Store at -20°C. Avoid repeated freeze/thaw cycles

Antigen Description

Vitamin B12 is a member of the vitamin B complex. It contains cobalt, and so is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in meat, eggs and dairy products. There has been considerable research into proposed plant sources of vitamin B12. Fermented soya products, seaweeds, and algae such as spirulina have all been suggested as containing significant B12. However, the present consensus is that any B12 present in plant foods is likely to be unavailable to humans and so these foods should not be relied upon as safe sources. Vitamin B12 is necessary for the synthesis of red blood cells, the maintenance of the nervous system, and growth and development in children. Deficiency can cause anaemia. Vitamin B12 neuropathy, involving the degeneration of nerve fibres and irreversible neurological damage, can also occur.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Carbon dots as fluorescent probes for detection of VB12 based on the inner filter effect

RSC ADVANCES

Authors: Sun, X. Y.; Yuan, M. J.; Liu, B.; Shen, J. S.

Abstract

In this study, we constructed a new fluorescent sensing for VB12 and investigated the mechanism of vitamin B-12 (VB12) quenching fluorescence of carbon dots (CDs). The fluorescence suppression is attributed to the inner filter effect (IFE) because of the overlap between UV-vis absorption spectrum of VB12 and emission/excitation spectra of CDs. This CDs-based sensor provides obvious advantages of simplicity, convenience, rapid response, high selectivity and sensitivity, which has potential application for the detection of VB12 in the medical and food industry.

Dual-wavelength Overlapping Resonance Rayleigh Scattering Spectra of the Interactions of Vitamin B-12 with 12-Tungstophosphoric Acid and Their Analytical Applications

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE

Authors: Cui Zhi-Ping; Liu Shao-Pu; Liu Zhong-Fang; Hu Xiao-Li

Abstract

A new dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect vitamin B-12(VB12) with 12-tungstophosphoric acid(TP). In a HCl medium(pH=1.0), VB12 could react with TP to form a 3 : 1 ion-association complex. As a result, the new spectra of DWO-RRS, second order scattering(SOS) and frequence doubling scattering(FDS) appeared and their intensities were enhanced greatly. Their maximum wavelengths were located at 330 nm(RRS), 370 nm(RRS), 608 nm(SOS) and 386 nm(FDS), respectively. The three kinds of scattering intensities(Delta I-RRS, Delta I-SOS and Delta I-FDS) were proportional to the concentration of VB12 in certain ranges. The methods had high sensitivity with the detection limit of 2.0-7.6 ng/mL. The optimal conditions and the effects of coexistent substances of RRS method were investigated. So, a simple, rapid and new method had been proposed to determine trace VB12. It had been applied to the determination of VB12 in urine samples and vitamin B-12 tablets with satisfactory results. In addition, the reasons for RRS enhancement and composition of ion-association complex were discussed.