Vitamin B-12 ELISA Kit

Sample

blood, serum

Species Reactivity

Human

Detection Method

cELISA

Intended Use

The Quantitative Determination of Vitamin B-12 Concentration in Human Serum by a Microplate Enzyme Immunoassay, Colorimetric.

Storage

Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.

Performance Characteristics

Accuracy:
The Vitamin B12 ELISA Test System was compared with a reference method. Biological specimens from low, normal and relatively high Vitamin B12 level populations were used (the values ranged from 156 pg/ml-1830 pg/ml). The total number of such specimens was 56. The least square regression equation and the correlation coefficient were computed for this Vitamin B12 test in comparison with the reference method. The data obtained is displayed in Table.

Only slight amounts of bias between this method and the reference method are indicated by the closeness of the mean values. The least square regression equation and correlation coefficient indicates excellent method agreement.

Precision

The within and between assay precision of the Vitamin B12 ELISA Test System were determined by analyses on three different levels of pool control sera.The number, mean values, standard deviation and coefficient of variation for each of these control sera are presented in Tables.


*As measured in ten experiments in duplicate over a ten day period.

Sensitivity

The Vitamin B12 ELISA Test System has a sensitivity of 70.13 pg/ml. The sensitivity was ascertained by determining the variability of the 0 pg/ml serum calibrator and using the 2σ(95% certainty) statistic to calculate the minimum dose.

General Description

Vitamin B-12 is one of the nine water soluble vitamins important for healthy body functioning. The most important roles Vitamin B-12 plays in the human body are in the formation of red blood cells and the formation of the myelin sheath around the nerves. Since the effects are seen in body systems with a large range of function, the symptoms of Vitamin B-12 deficiency can sometimes be very ambiguous. A deficiency may also take from months to years to manifest depending on the cause and severity. Two of the most common causes of Vitamin B-12 deficiency are diet and age. Because most sources of dietary Vitamin B-12 come from animals, vegans who do not efficiently supplement their diet are at risk. The elderly community is also at high risk because of their diet, as well as the less efficient functioning of their digestive system. Intake of Vitamin B-12 starts by ingestion and then digestion by saliva. Once reaching the gut, Vitamin B-12 bound to proteins in food are released by the acids present. The B12 can then bind the Intrinsic factor. Once bound to IF, Vitamin B-12 is stable enough to travel into the intestines where it can be absorbed into your body through of its association with IF. Two very useful tests to distinguish between Vitamin B-12 deficiency and folate deficiency are methylmalonyl CoA (MMA) and homocysteine (hcy). Both deficiencies are represented by similar symptoms; however, even though both show increased levels of homocysteine, only Vitamin B-12 deficiency causes an increase in methylmalonyl CoA. The increase in levels of methylmalonyl CoA and homocysteine is thought to be the root cause of any symptoms that accompany a Vitamin B-12 deficiency. High levels of these two analytes in the blood stream causes increased oxidative stress to cells therefore causing increased apoptosis. In turn, vascular disease results in the form of atherosclerosis, coronary heart disease and/or neurodegeneration.

Standard Curve

*The above data and table below is for example only. Do not use it for calculating your results

Note: Multiply the horizontal values by 0.738 to convert into pM/ml.