Food Vitamin B12 ELISA Kit (DEIA2541)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
multivitamin tablets, capsules, multivitamin juices, multivitamin jam, grain products, multivitamin sweets
Species Reactivity
N/A
Contents of Kit
1. Microtiter plate consisting of 12 strips with 8 breakable wells each, coated with anti-vitamin B12.
2. Vitamin B12 Standards (0; 0.4; 1; 4; 10; 40 ng/mL)
3. Conjugate (Vitamin B12-Peroxidase)
4. Substrate Solution (TMB)
5. Stop Solution (0.5 M H2SO4)
6. Sample Diluent (PBS)
7. Washing Solution (PBS + Tween 20)
Storage
Store the kit at 2-8°C in a refrigerator. For more detailed information, please download the following document on our website.
Detection Range
0-40 ng/mL
Sensitivity
0.3 ng/mL

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References


Effects of vitamin B-12 on bright light on cognitive and sleep-wake rhythm in Alzheimer-type dementia

PSYCHIATRY AND CLINICAL NEUROSCIENCES

Authors: Ito, T; Yamadera, H; Ito, R; Suzuki, H; Asayama, K; Endo, S

The present study investigated the effects of vitamin B-12 (VB12) On circadian rhythm in Alzheimer-type dementia (ATD). Twenty-eight ATD patients were treated with bright light therapy (BLT) for 8 weeks. For the latter 4 weeks. half were treated with VB12 with BLT (BLT + VB12). We evaluated the cognitive state with Mini-Mental State Examination and the circadian rhythm with actigraphy after the fourth and eighth week. After the first 4 weeks BLT improved the circadian rhythm disturbances and cognitive state especially in the early stage of ATD. Although the latter 4 week-BLT caused no significant effects on the circadian rhythm: BLT+ VB12 improved the vigilance level during the daytime. These results suggest that VB12 has some efficiency to enhance vigilance for ATD patients.

Effects of aldose reductase inhibitor and vitamin B-12 on myocardial uptake of iodine-123 metaiodobenzylguanidine in patients with non-insulin-dependent diabetes mellitus

EUROPEAN JOURNAL OF NUCLEAR MEDICINE

Authors: Utsunomiya, K; Narabayashi, I; Tamura, K; Nakatani, Y; Saika, Y; Onishi, S; Kariyone, S

This study was undertaken to examine the effects of aldose reductase inhibitor (ARI) and vitamin B-12 (VB12) on myocardial uptake of iodine-123 metaiodobenzylguanidine (MIBG) in patients with diabetic autonomic disorder. Myocardial scintigraphy using I-123- MIBG was performed on 20 healthy volunteers (controls) and 56 patients with non-insulin-dependent diabetes mellitus (NIDDM), in order to obtain the heart/mediastinum ratio in the initial (HMi) and the delayed images (HMd), and the washout rate (%WR). Thirty-four of the 56 NIDDM patients could be diagnosed as having diabetic autonomic disorder by evaluating their scintigraphic findings in comparison with the controls. Seventeen of these 34 patients received 150 mg/day of epalrestat (ARI group) in three divided doses before meals, and the other 17 received 1.5 mg/day of mecobalamin (VB12 group) in three divided doses after meals, for 3-5 months. According to the presence or absence of clinical symptoms of autonomic or peripheral somatic nerve disorder, the patients were subclassified into four groups. group 1=patients, with autonomic symptoms or somatosensory disorder in the ARI group; group 2=patients without autonomic symptoms or somatosensory disorder in the ARI group; group 3=patients with autonomic symptoms or somatosensory disorder in the VB12 group; and group 4=patients without autonomic symptoms or somatosensory disorder in the VB12 group. After completion of the treatment, myocardial scintigraphy was performed again. Comparing the results obtained before and after the treatment, it was seen that ARI improved only the HMi in group 1 (P=0.046), whereas VB12 significantly improved HMi in the group 3 (P=0.018) and HMI, HMd and %WR in group 4 (P=0.043, P=0.018 and P=0.043, respectively). We conclude that VB12 is more efficacious than ARI in the treatment of diabetic cardiovascular autonomic disorder.

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