Testosterone ELISA Kit

Sample

Serum and plasma

Species Reactivity

Human

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for the Quantitative Determination of Testosterone in Plasma and Serum.

Contents of Kit

The kit contains reagents for 96 determinations. They have to be stored at 2-8°C. Expiry data are found on the labels of the bottles and the outer package.
1. Microtiter plate consisting of 12 strips with 8 breakable wells each, coated with antitestosterone.
2. Testosterone Standards (0; 100; 500; 1000; 5000; 15000 pg/mL): 1.0 mL each, dyed red, ready-to-use.
3. Conjugate (Testosterone-Peroxidase): 100 μL as 100× concentrate, dyed red. Dilute 1:100 with conjugate diluent. The diluted conjugate is not stable and cannot be stored.
4. Conjugate Diluent: 6 mL, dyed red, ready-to-use.
5. Substrate Solution (TMB): 15 mL; ready-to-use.
6. Stop Solution (0.5 M H₂SO₄): 15 mL; ready-touse.
7. Sample Diluent (PBS): 2 × 50 mL; dyed red, ready-to-use.
8. Washing Solution (PBS + Tween 20): 30 mL as 10× concentrate, dyed blue. Dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.
9. Two plastic foils to cover the strips during the incubation.
10. Plastic bag to store unused microtiter strips.
11. Instruction Manual.

Storage

Store kit at 2-8°C. It is stable up to the expiry date stated on the label of the box. Do not use kit beyond its expiry date.

Precision

The intra-assay variation of the testosterone test was determined to 3%.

Sensitivity

The sensitivity of the Testosterone test is 100 pg/ mL (based on the standard curve).

General Description

The steroid hormone testosterone belongs to the androgens, which are produced in the testicles, adrenal cortex, and in small quantities also in the ovary. Besides testosterone, androstenedione and androsterone are of importance in this group. The androgens stimulate the development of male sexual organs as well as the differentiation of the secondary male sex characteristics. Steroid hormones are used since some decades to enhance the growth of cattle and other animals. Hormones employed can be endogenous ones (testosterone, progesterone, estradiol) or synthetic products. Since the hormones show side-effects when ingested with the meat, e.g. genotoxic, neurobiologic and carcinogenic effects, the risks for the consuming persons should be estimated. In contrast to the USA, where the use of sex hormones as implantates is allowed, in the European Union all substances, which lead to an enhancement of natural growth, are forbidden (88/146/EEC and 96/22/EC). These guidelines imply an obligation to monitor these hormones and their metabolites in animals and animal products. Control methods like special gaschromatographic procedures have been established. The ELISA belongs to the most sensitive procedures and can detect residues down to 0.1 ng/mL (100 ppt).