Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit

Sample

serum, plasma, tissue homogenates

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the quantitative determination of human low density lipoproteinreceptor (LDLR) concentrations in serum, plasma, tissue homogenates.

Contents of Kit

1. Assay plate (12 × 8 coated Microwells), 1(96 wells)
2. Standard (Freeze dried), 2
3. Biotin-antibody (100× concentrate), 1 × 120 μl
4. HRP-avidin (100× concentrate), 1 × 120 μl
5. Biotin-antibody Diluent, 1 × 15 ml
6. HRP-avidin Diluent, 1 × 15 ml
7. Sample Diluent, 1 × 50 ml
8. Wash Buffer (25× concentrate), 1 × 20 ml
9. TMB Substrate, 1 × 10 ml
10. Stop Solution, 1 × 10 ml
11. Adhesive Strip (For 96 wells), 4
12. Instruction manual, 1

Storage

Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit:
Coated assay plate: May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
Standard/Biotin-antibody/HRP-avidin: May be stored for up to 1 month at 2 - 8° C. If don' t make recent use, better keep it store at -20°C.
Biotin-antibody Diluent/HRP-avidin Diluent/Sample Diluent/Wash Buffer/TMB Substrate/Stop Solution: May be stored for up to 1 month at 2 - 8°C.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate toassess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

31.25 pg/ml-2000 pg/ml.

Sensitivity

The minimum detectable dose of human LDLR is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

General Description

The low-density lipoprotein receptor (LDL-R) is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide)that mediates the endocytosis of cholesterol-rich low-density lipoprotein (LDL). It is a cell-surface receptor that recognizes apolipoprotein B100 (ApoB100), which is embedded in the outer phospholipid layer of very low-density lipoprotein (VLDL), their remnants—i.e. intermediate-density lipoprotein (IDL), and LDL particles. The receptor also recognizes apolipoprotein E (ApoE) which is found in chylomicron remnants and IDL. In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It belongs to the low density lipoprotein receptor gene family. It is most significantly expressed in bronchial epithelial cells and adrenal gland and cortex tissue.
Michael S. Brown and Joseph L. Goldstein were awarded the 1985 Nobel Prize in Physiology or Medicine for their identification of LDL-R and its relation to cholesterol metabolism and familial hypercholesterolemia. Disruption of LDL-R can lead to higher LDL-cholesterol as well as increasing the risk of related diseases. Individuals with disruptive mutations (defined as nonsense, splice site, or indel frameshift) in LDLR have an average LDL-cholesterol of 279 mg/dL, compared with 135 mg/dL for individuals with neither disruptive nor deleterious mutations. Disruptive mutations were 13 times more common in individuals with early-onset myocardial infarction or coronary artery disease than in individuals without either disease.