Sample
cell culture supernatants, tissues homogenates, serum, plasma
Detection Method
Sandwich ELISA
Intended Use
For the quantitative determination of mouse Low Density Lipoprotein Receptor (LDL R) concentrations in cell culture supernates, tissue homogenates, serum, and plasma.
Contents of Kit
1. Mouse LDL R Microplate
2. Mouse LDL R Standard
3. Mouse LDL R Control
4. Mouse LDL R Conjugate
5. Assay Diluent
7. Wash Buffer Concentrate(25X)
8. Color Reagent A
9. Color Reagent B
10. Stop Solution
Storage
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Precision
Intra-assay Precision:
4.6-5.3%
Inter-assay Precision:
5.7-8.1%
Detection Range
0.008-0.064 ng/mL
Citations
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Grosso, D; Pereira, R; et al. Antibodies against electronegative LDL inhibit atherosclerosis in LDLR-/- mice. FREE RADICAL BIOLOGY AND MEDICINE 39:S150-S150(2005).
Ason, B; van der Hoorn, JWA; et al. PCSK9 inhibition fails to alter hepatic LDLR, circulating cholesterol, and atherosclerosis in the absence of ApoE. JOURNAL OF LIPID RESEARCH 55:2370-2379(2014).
COA
Certificate of Analysis
