Human Renin [His]

Product Overview

RecombinantHuman Renin contains a 8X-Histidine tag at C-terminus and has a totalmolecular weight of 43,725 Da, was expressed in HEK cells, which is fullyactivatable by catalytic amounts of trypsin.

Nature

Recombinant

Tag/Conjugate

His

Format

Purified, Liquid

Concentration

Lot specific(OD280nm, E0.1% = 1.09)

Buffer

50mMTris, pH 8.0

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction. It regulates blood pressure and electrolyte homoeostasis. Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. Renin has been identified to be an attractive target for the treatment of hypertension. The Amplite Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and renin activity using our proprietary Tide Fluor 3 (TF3)/Tide Quencher 3 (TQ3) fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, and the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay. With the Amplite Renin Assay Kit, we have detected as little as 1ng renin in a 100 L reaction volume.

Antigen Description

Reninalso known as an angiotensinogenase, is an enzyme that participates in thebody"s renin-angiotensin system (RAS) -- also known as theRenin-Angiotensin-Aldosterone Axis -- that mediates extracellular volume(i.e., that of the blood plasma, lymph and interstitial fluid), and arterialvasoconstriction. Thus, it regulates the body"s mean arterial blood pressure.

Keywords

REN; renin; HNFJ2; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme