Detection Method
Sandwich ELISA
Intended Use
The product is an enzyme immunoassay for measurement of active Renin in human serum and plasma.
Contents of Kit
1. Microtiter wells, 12 x 8 ( break apart ) strips, 96 wells
2. Standard ( Standard 0-5 ), 6 vials
3. Control Low and High, 2 vials
4. Assay Buffer, 1 vial, 20 mL
5. Enzyme Conjugate, 1 vial, 14 mL
6. Substrate Solution, 1 vial, 14 mL
7. Stop Solution, 1 vial, 14 mL
8. Wash Solution, 1 vial, 30 mL
Storage
Store all contents at 2-8°C. For more detailed information, please download the following document on our website.
Detection Range
2.5-128 pg/mL
Citations
Publication ()
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Validated low-volume immunoassay for the reliable determination of direct renin especially valuable for pediatric investigations
J. Schaefer, B. B. Burckhardt, J. Tins, A. Bartel & S. Laeer
Journal of Immunoassay and Immunochemistry2017 JulPubMed ID: 28699839Read Article
Applications: ELISA
Reactive species: Human
"Abstract: Introduction: The pharmacotherapy of pediatric patients suffering from heart failure is extrapolated from adults due to missing data in children. Objectives: Development and validation of a low-volume immunoassay for the reliable determination of renin. Experimental: The immunoassay was validated according to international guidelines. Results: The assay allows the reliable determination of renin in 40 μL plasma within a calibration range of 4-128 pg/mL. Between-run accuracy varied from -3.3 to +3.0% (relative error), while between-run precision ranged from 4.9 to 11.3% (coefficient of variation). Conclusion: The low-volume immunoassay facilitates the reliable collection of pharmacodynamic data in children."
Article snippet: [6] Human Renin ELISA Kit (Cat. No. DElA2168) by Creative Diagnostics, http://img.creativediagnostics.com/pdf/DElA2168,REN.pdf (last access on January 5t, 2017)
Figure 1. Time-dependent renin concentrations of a 30-year-old female healthy volunteer over a period of 12 hours.