Intended Use
This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of total rat prorenin/renin in biological fluid.
Contents of Kit
1. Coated Plate
2. Standard
3. Wash Buffer
4. Substrate
5. Primary Antibody
6. Avidin HRP
Storage
1. Store this kit and its components at 4°C until use.
2. Reconstituted standards and primary may be stored at -70°C for later use. DO NOT freeze/thaw the Standards and Primary Antibody more than once.
Detection Range
Typical rat prorenin levels range from 0 to 400 ng/mL. The assay range for this ELISA is 0.1 to 100 ng/mL. Samples with prorenin levels above 100 ng/mL should be diluted in Blocking Buffer and retested.
Citations
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Chen, X; Qiu, ZH; et al. Effectiveness and Safety of a Therapeutic Vaccine Against Angiotensin II Receptor Type 1 in Hypertensive Animals. HYPERTENSION 61:408-+(2013).
Wang, RQ; Zagariya, A; et al. Fas-induced apoptosis of alveolar epithelial cells requires ANG II generation and receptor interaction. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 277:L1245-L1250(1999).
COA
Certificate of Analysis
