Proinsulin ELISA Kit

Name: Proinsulin ELISA Kit
SKU: DEIA2247
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Proinsulin ELISA is an enzyme immunoassay for the quantitative measurement of Proinsulin (intact) in serum and plasma.

Contents of Kit

1. Microtiter wells, 12 x 8 (break apart) strips, 96 wells
2. Standard (Standard 0-5), 6 vials (lyophilized), 1 mL
3. Control (low and high)
4. Sample Diluent, 1 vial, 2 mL
5. Enzyme Conjugate 11X concentrate, 1 vial, 1.2 mL
6. Conjugate Diluent, 1 vial, 12 mL
7. Assay Buffer, 1 vial, 12 mL
8. Substrate Solution, 1 vial, 14 mL
9. Stop Solution, 1 vial, 14 mL
10. Wash Solution, 1 vial, 30 mL (40X concentrated)

Storage

When stored at 2-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. Opened reagents must be stored at 2-8°C. Microtiter wells must be stored at 2-8°C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for two months if stored as described above. For more detailed information, please download the following document on our website.

Detection Range

0.5 pmol/L-66 pmol/L.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Metabolomics Study Reveals Inhibition and Metabolic Dysregulation inStaphylococcus aureusPlanktonic Cells and Biofilms Induced by Carnosol

FRONTIERS IN MICROBIOLOGY

Authors: Shen, Fengge; Ge, Chunpo; Yuan, Peng

Abstract

Staphylococcus aureus(S. aureus) is a global health threat accompanied by increasing in drug resistance. To combat this challenge, there is an urgent need to find alternative antimicrobial agents againstS. aureus. This study investigated the antimicrobial efficacy of carnosol againstS. aureususing anin vitromodel. The effects of carnosol were determined based on the antimicrobial effects or formation and disruption of biofilms. Finally, metabolomics ofS. aureusgrown as planktonic cells and biofilms with carnosol treatment were analyzed using gas chromatography-mass spectrometry. The minimum inhibitory concentrations (MICs) of carnosol were 32 to 256 mu g/mL against the sixteen testedS. aureusstrains. Among the biofilms, we observed a reduction in bacterial motility of theS. aureus, biofilm development and preformed biofilm after carnosol treatment. Moreover, the significantly altered metabolic pathways upon carnosol treatment inS. aureusplanktonic cells and biofilms were highly associated with the perturbation of glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, arginine biosynthesis, and aminoacyl-tRNA biosynthesis. In addition, glutathione metabolism, D-glutamine and D-glutamate metabolism were significantly changed in the biofilms. This study establishes the antibacterial and antibiofilm properties of carnosol, and will provide an alternative strategy for overcoming the drug resistance ofS. aureus.

Virtual labs and simulations: Where to find them and tips to make them work

PHYSICS TEACHER

Authors: Lincoln, James

Abstract

In this article I focus on ready-to-go online labs with no plug-ins or additional software required; students just go straight to the website. This means no Flash, no Java, no apps, but yes, HTML5 and JavaScript. Then teachers can deliver their instructions (perhaps over video chat). Below I have listed my personal favorite simulated lab websites, with a more extended list appearing in the online appendix to this article. My personal criteria for a good lab simulation would be one that gives the feeling that you are performing the actual experiment (Fig. 1). It should be immersive, engaging, and require almost no instruction to take the data.