Rat Anti-Human IgG Fc Monoclonal Antibody, clone N2421H16

Cis-diol compounds are class of biomolecules including nucleosides, glycoproteins, saccharides, and nucleotides, which play vital roles in various biological processes. Due to low abundances of these species in the complex biological samples, their identification and analysis is difficult. Boronate affinity materials are commonly used for the isolation and enrichment of cis-diol compounds, due to their unique, facile and selective enrichment mechanism. In this study we report a selective approach to extract nucleosides, glycopeptides and glycoproteins using boronic acid functionalized GMA-MAA-DVB polymer. This novel polymer, reported for the first time in proteomics, have high BET surface area (132.8447 m(2) g(-1)) which contribute to efficient enrichment and average pore size (20.3449 nm) to facilitates the nano confinement effect for strong interactions. Hydrophilic character of methacrylic acid and diethylenetriamine, along with inherent affinity of boronic acid for glycosylated biomolecules result in selectivity up to 1:500 for peptides and 1:1000 for glycoprotein. Binding constant for cis-diol compounds are in the range of 10(-4) to 10(-6) M and theoretical binding capacity up to 85 mg g(-1) for HRP and 180 mg g(-1) for IgG, respectively. Furthermore, boronic acid functionalized polymer (BFP) enrich glycoproteins and glycopeptides in range of 1 pg mL(-1) and 0.04 ng mL(-1) with S/N >= 3. Finally, material is applied to enrich the glycoproteins from healthy human saliva sample and six glycoproteins are identified.

Pulmonary tuberculosis (TB) is one of the common risk factors for chronic pulmonary aspergillosis (CPA). A positive Aspergillus IgG is a key element of the diagnosis of CPA but this has not been studied in Indonesia. We conducted studies with patients at the end of TB therapy in Indonesia. We performed receiver operating curve (ROC) analysis to determine the optimum cutoff of the Aspergillus-specific IgG level (Immulite and Dynamiker ELISA) in those patients who met criteria of CPA in relation to control groups. In 203 TB patients, 26 (13%) patients had clinical and radiological features of CPA. We derived optimum cutoffs for Immulite Aspergillus-specific IgG of 11.5 mg/L and Dynamiker anti-galactomannan IgG of 106.8 AU/mL (sensitivity 89% and 83%, specificity 78% and 51%, respectively). The currently accepted Aspergillus-specific IgG cutoff of Immulite and Dynamiker assays for CPA diagnosis may require slight adjustment for the Indonesian population. (C) 2020 Elsevier Inc. All rights reserved.