Immunoglobulin G ELISA Kit (DEIA084J)

Regulatory status: For research use only, not for use in diagnostic procedures.

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biological sample
Species Reactivity
Intended Use
The kit is a highly sensitive twosite enzyme linked immunoassay (ELISA) for measuring IgG in Human biological samples.
Contents of Kit
1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5x concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20x concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100x One vial containing 150μL of affinity purified anti-Human IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3' ,5,5' tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN IgG ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgG.
7. HUMAN IgG CALIBRATOR One vial containing Human IgG calibrator.
All reagents provided are stored at 2-8°C. Refer to the expiration date on the label.
Reconstitution And Storage
1. DILUENT The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8°C.
2. WASH SOLUTION The 20X Wash Solution Concentrate


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