Human Furin ELISA Kit (DEIA10900)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatants, tissues lysates, cell lysates
Species Reactivity
Human
Intended Use
The Human Furin ELISA Kit is suitable for the quantitative detection of Human Furin in cell culture supernates, tissue lysates and cell lysates.
Contents of Kit
1. 96-well plate precoated with anti-human Furin antibody: 1
2. Lyophilized recombinant human Furin Standard: 20 ng/tube x 2
3. Biotinylated anti-human Furin antibody: 130 μL (dilution 1: 100)
4. Avidin-Biotin-Peroxidase Complex (ABC): 130 μL (dilution 1: 100)
5. Sample diluent buffer: 30 mL
6. Antibody diluent buffer: 12 mL
7. ABC diluent buffer: 12 mL
8. TMB color developing agent: 10 mL
9. TMB stop solution: 10 mL
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Detection Range
312 pg/mL-20,000 pg/mL
Standard Curve

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References


Kam, YW; Lum, FM; et al. Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 glycoprotein. EMBO MOLECULAR MEDICINE 4:330-343(2012).
Metz, SW; Geertsema, C; et al. Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells. VIROLOGY JOURNAL 8:-(2011).

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