Human EBV EBNA1 IgG ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Quantitative and qualitative tests for detection of human anti-Epstein-Barr Virus antibodies in serum or plasma.

Contents of Kit

1.Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96) MTP, 1 frame, the coating material is inactivated, 12
2.Standard serum (ready-to-use) STD Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, colouring: Amaranth O, 2 x 2 ml
3.Negative control serum (ready-to-use) NEG Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, colouring: Lissamin green V, 2 ml
4.Anti-human-IgG-conjugate (ready-to-use) APC Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution, preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane, 13 ml
5.Washing solution concentrate (sufficient for 1 litre) WASH Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide, 33.3 ml
6.Dilution buffer DILB Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt, 2 x 50 ml
7.Stopping solution STOP 1.2 N sodium hydroxide, 15 ml
8.Substrate (ready-to-use) pNPP Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!), 13 ml
9.Quality control certificate with standard curve and evaluation table INFO (quantification of antibodies in IU/ml or U/ml), 1

Storage

General Description

Epstein-Barr Virus (EBV), a DNA virus, is a member of the Human Herpesvirus group and is pathogenic for humans.
EBV transmission takes place primarily via the saliva of infected individuals. Transfer of the virus via blood, blood products and bone marrow transplants have also been reported, but this mode of transmission is comparatively rare.
On primary infection, the cells of the salivary gland are infected first. At this stage of infection respiratory symptoms are very common. Due to the subsequent infection of B cells in the adjacent lymphoid tissue, the virus spreads throughout the body. Infection of B cells results in a polyclonal stimulation of lymphoproliferation which is normally controlled by the immune system. In the later course of infection, high fever, splenomegaly, lymphadenitis, thrombocytopenia and hepatitis may be observed. The disease resulting from primary infection is called infectious mononucleosis (IM) or glandular fever. Since viral transmission principally happens by oral contact, the primary infection has also been called "kissing disease". In rare cases acute mononucleosis may progress to a chronic disease, and reactivation of EBV has been observed in immunosuppressed patients.
EBV is closely associated with nasopharyngeal carcinoma, and Burkitt lymphoma (BL), is at least partly correlated with EBV. This B cell tumor of monoclonal origin is endemically clustered in tropical regions of Africa and Asia. The geographical distribution of BL correlates with that of malaria. Since it is believed that infections with Plasmodium have some influence on the immune system, it is suggested that malaria might be an important cofactor for the development of Burkitt lymphoma in these regions.
Due to the complex structure of the virus, the tight regulation of the viral life cycle, and the appearance of latent or productive infections, the antibody response seen after EBV infection may be quite complex and therefore difficult to interpret.