Sample
cell lysates, serum, plasma
Detection Method
Sandwich ELISA
Intended Use
The Granzyme B (Mouse) ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Mouse Granzyme B concentrations within any experimental sample including cell lysates, serum and plasma.
Contents of Kit
1. Microstrips Coated w/ Capture Antibody, 12 x 8-Well Microstrips.
2. Protein Standard, Lyophilized (83ng), Red.
3. Biotinylated Detection Antibody, Lyophilized, Yellow.
4. 400x Streptavidin-HRP, 30 μL, Blue.
5. Wash Buffer (10x), 50 mL, Clear.
6. Assay Diluent, 50 mL, Clear.
7. Ready-to-Use Substrate, 12 mL, Brown.
8. Stop Solution, 12 mL, Clear.
9. Adhesive Plate Sealers, 4 Sheets.
10. Technical Manual, 1 Manual.
Detection Range
40-5000 pg/mL
Citations
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Crosby, EJ; Goldschmidt, MH; et al. Engagement of NKG2D on Bystander Memory CD8 T Cells Promotes Increased Immunopathology following Leishmania major Infection. PLOS PATHOGENS 10:-(2014).
Urbanellis, P; Shyu, W; et al. The regulatory T cell effector molecule fibrinogen-like protein 2 is necessary for the development of rapamycin-induced tolerance to fully MHC-mismatched murine cardiac allografts. IMMUNOLOGY 144:91-106(2015).
COA
Certificate of Analysis
