Species Reactivity
Human, Mouse, Rat
Intended Use
The NMDAR1 (Phospho-Ser896) Colorimetric Cell-Based ELISA Kit allows for the qualitative detection of NMDAR1 (Phospho-Ser896) and the effects that certain stimulation conditions have on NMDAR1 (Phospho-Ser896) expression in different cell lines.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 10x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-NMDAR1 (Phospho-Ser896) Antibody (Rabbit Polyclonal) 60 μL (100x) Red.
7. 100x Anti-NMDAR1 Antibody (Rabbit Polyclonal) 60 μL (100x) Purple.
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 μL (100x) Green.
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 mL (1x), Glass.
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 mL (1x), Glass.
11. Primary Antibody Diluent, 12 mL (1x), Clear.
12. Ready-to-Use Substrate, 12 mL (1x), Brown.
13. Stop Solution, 12 mL (1x), Clear.
14. Crystal Violet Solution, 12 mL (1x), Glass.
15. SDS Solution, 24 mL (1x), Clear.
16. Adhesive Plate Seals, 4 Seals.
Citations
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Chen, LT; Gilman, AG; et al. A candidate target for G protein action in brain. JOURNAL OF BIOLOGICAL CHEMISTRY 274:26931-26938(1999).
Demontis, D; Nyegaard, M; et al. Association of GRIN1 and GRIN2A-D With Schizophrenia and Genetic Interaction With Maternal Herpes Simplex Virus-2 Infection Affecting Disease Risk. AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS 156B:913-922(2011).