Anti-Pyruvate Kinase (native) polyclonal antibody

In this study, we determined whether the calcium-dependent protein kinase (PKC) signaling pathway is involved in the improvement of fear memory reconsolidation induced by the intrahippocampal administration of spermidine in rats. Male Wistar rats were trained in a fear conditioning apparatus using a 0.4-mA footshock as an unconditioned stimulus. Twenty-four hours after training, animals were re-exposed to the apparatus in the absence of shock (reactivation session). Immediately after the reactivation session, spermidine (2-200 pmol/site), the PKC inhibitor 3-[1-(dimethylaminopropyl) indol-3-yl]-4-(indol-3-yl) maleimide hydrochloride (GF 109203X, 0.3-30 pg/site), the antagonist of the polyamine-binding site at the NMDA receptor, arcaine (0.2-200 pmol/site), or the PKC activator phorbol 12-myristate 13-acetate (PMA, 0.02-2 nmol/site) was injected. While the post-reactivation administration of spermidine (20 and 200 pmol/site) and PMA (2 nmol/site) improved memory reconsolidation, GF 109203X (1, 10, and 30 pg/site) and arcaine (200 pmol/site) impaired it. GF 109203X (0.3 pg/site) impaired memory reconsolidation in the presence of spermidine (200 pmol/site). PMA (0.2 nmol/site) prevented the arcaine (200 pmol/site)-induced impairment of memory reconsolidation. Anisomycin (2 mu g/site) also impaired memory reconsolidation in the presence of spermidine (200 pmol/site). Drugs had no effect when they were administered in the absence of reactivation. These results suggest that the spermidine-induced enhancement of memory reconsolidation involves PKC activation.

This research aims to develop a training model on installation and maintenance of electrical installations homes for youth in the village. The research using activities carried out by using participatory approaches demand responsive approach method. Training model development is based on the needs and potential of the community and local village in the context of development and sustainability, the goal of this training can provide job opportunities for the youth in an effort to reduce unemployment. Skills training model development include: (1) the planning stage (need assessment), (2) the implementation stage, (3) mentoring phase and (4) monitoring and evaluation. The results of the training model of the installation of electrical developers are opening business opportunities for the youth in electrical installation so as to improve the standard of living and well-being as well as the establishment of a model village built supported by the village government officials with PKM team-based partnership with UPI. PKM activity guided village-based partnership was attended by about 35 youth who are representatives of each pillar citizens to be given training and assistance in the installation and maintenance of residential electrical installation. The output of Entrepreneurship PKM is the emergence of a cadre of independent village installer from among the youth who mastering the skills of installation and maintenance of electrical installations of residential houses. Another outcome of this Patronage village PKM activity is the publication of articles or scientific publications in national or international journal.