Mouse Tumor type M2 Pyruvate Kinase ELISA Kit

This research aims to develop a training model on installation and maintenance of electrical installations homes for youth in the village. The research using activities carried out by using participatory approaches demand responsive approach method. Training model development is based on the needs and potential of the community and local village in the context of development and sustainability, the goal of this training can provide job opportunities for the youth in an effort to reduce unemployment. Skills training model development include: (1) the planning stage (need assessment), (2) the implementation stage, (3) mentoring phase and (4) monitoring and evaluation. The results of the training model of the installation of electrical developers are opening business opportunities for the youth in electrical installation so as to improve the standard of living and well-being as well as the establishment of a model village built supported by the village government officials with PKM team-based partnership with UPI. PKM activity guided village-based partnership was attended by about 35 youth who are representatives of each pillar citizens to be given training and assistance in the installation and maintenance of residential electrical installation. The output of Entrepreneurship PKM is the emergence of a cadre of independent village installer from among the youth who mastering the skills of installation and maintenance of electrical installations of residential houses. Another outcome of this Patronage village PKM activity is the publication of articles or scientific publications in national or international journal.

Northwestern Pakistan is the leading seed potato-producing area of the country. Potato cultivars grown commercially in the region were screened against Potato mop-top virus (PMTV) under screenhouse conditions and in naturally infested fields during 2010-2014. The potato cultivars, Asterix, Cardinal, Desiree, Diamant, Kuroda, Paramount, Raja, Rocco, Sarpomira, and Ultimas, were grown in viruliferous soil in a bait test and were all found to be susceptible to PMTV (two isolates from Pakistan-Hazara (PMTV-PkH) and Pakistan-Malakand (PMTV-PkM)) in screenhouse experiments. The virus was detected in all cultivars as tested by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) in leaf, root, and tuber samples, and by back inoculation of sap from roots of bait plants on indicator plants. The ELISA values (A(405nm)) were lower in leaf, root, and tuber samples in cultivar Desiree compared to the other cultivars, whereas in the infectivity assay, the virus was recovered in all cultivars and exhibited characteristic symptoms on diagnostic hosts. All 10 potato cultivars were further tested against PMTV in previously known infested fields at Baffa, Hazara division, and Kalam, Malakand division, in northwestern Pakistan. The cultivars tested at both sites were found susceptible to PMTV. The virus was detected by DAS-ELISA and RT-PCR in randomly selected potato tubers of each cultivar during 3 years at both sites. We conclude that commercially grown potato cultivars in northwestern Pakistan are susceptible (moderately sensitive to sensitive) to PMTV, requiring a comprehensive potato germplasm screening program for the evaluation of sources of resistance against the virus.