Anti-Human PARP1 monoclonal antibody (CAB-7545RH)

Rabbit Anti-Human PARP1 monoclonal antibody for WB, ICC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TV1425
Species Reactivity
Human
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; FC: 1:50-1:100; ICC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Cleaved PARP on Jurkat cells lysates using anti-Cleaved PARP antibody at 1/1,000 dilution.
Fig2: Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1
Entrez Gene ID
UniProt ID

Citations


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References


Meng, XW; Koh, BD; et al. Poly(ADP-ribose) Polymerase Inhibitors Sensitize Cancer Cells to Death Receptor-mediated Apoptosis by Enhancing Death Receptor Expression. JOURNAL OF BIOLOGICAL CHEMISTRY 289:20543-20558(2014).
Casar, B; He, Y; et al. Blocking of CDCP1 cleavage in vivo prevents Akt-dependent survival and inhibits metastatic colonization through PARP1-mediated apoptosis of cancer cells. ONCOGENE 31:3924-3938(2012).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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