Anti-Human PARP1 monoclonal antibody (CAB-7545RH)
Rabbit Anti-Human PARP1 monoclonal antibody for WB, ICC, IP, FC
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Recommended Dilution: WB: 1:1,000-1:2,000; FC: 1:50-1:100; ICC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of Cleaved PARP on Jurkat cells lysates using anti-Cleaved PARP antibody at 1/1,000 dilution.
Fig2: Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1
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Olaussen, KA; Adam, J; et al. PARP1 impact on DNA repair of platinum adducts: Preclinical and clinical read-outs. LUNG CANCER 80:216-222(2013).
Alexander, BM; Wang, XZ; et al. DNA Repair Biomarkers Predict Response to Neoadjuvant Chemoradiotherapy in Esophageal Cancer. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 83:164-171(2012).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.