General Notes
This product (Catalog No. DPABH-00694) is a rabbit-derived polyclonal antibody raised against human PARP1, targeting the amino acid region 373–540 of the protein. This antibody exhibits cross-reactivity with Human PARP1 from mouse and rat. It has been affinity purified by Protein A. Poly(ADP-ribose) polymerase 1 (PARP1) is a multifunctional nuclear enzyme that serves as a primary sensor of DNA damage, catalyzing the transfer of ADP-ribose units from NAD⁺ to itself and other proteins in a post-translational modification process known as poly(ADP-ribosyl)ation (PARylation). This 113 kDa protein, encoded by the PARP1 gene on human chromosome 1, is essential for maintaining genomic integrity by facilitating various DNA repair pathways, including base excision repair (BER), nucleotide excision repair (NER), single-strand break repair (SSBR), and aspects of double-strand break repair such as non-homologous end joining (NHEJ) and homologous recombination (HR). Upon detecting DNA breaks via its N-terminal DNA-binding domain containing zinc fingers, PARP1 undergoes rapid activation, auto-PARylation, and recruitment of repair factors, thereby orchestrating the cellular response to genotoxic stress.
DPABH-00694 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:200), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-00694 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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