Anti-PTGS2 monoclonal antibody (DMAB5554RH)

Rabbit Anti-PTGS2 monoclonal antibody for WB, ICC/IF Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TD67-17
Species Reactivity
Human, Mouse
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549; Lane 2: THP-1
Fig2: ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Target


Alternative Names
COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase

Citations


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References


Dixon, RE; Hwang, SJ; et al. Chlamydia Infection Causes Loss of Pacemaker Cells and Inhibits Oocyte Transport in the Mouse Oviduct. BIOLOGY OF REPRODUCTION 80:665-673(2009).
Zahner, G; Schaper, M; et al. Prostaglandin EP2 and EP4 receptors modulate expression of the chemokine CCL2 (MCP-1) in response to LPS-induced renal glomerular inflammation. BIOCHEMICAL JOURNAL 422:563-570(2009).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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