Anti-PTGS2 monoclonal antibody (DMAB5554RH)
Rabbit Anti-PTGS2 monoclonal antibody for WB, ICC/IF
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Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution.
Lane 1: A549;
Lane 2: THP-1
Fig2: ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase
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Liszka, L; et al. DUCTAL ADENOCARCINOMA OF THE PANCREAS USUALLY RETAINED SMAD4 AND p53 PROTEIN STATUS AS WELL AS EXPRESSION OF EPITHELIAL-TO-MESENCHYMAL TRANSITION MARKERS AND CELL CYCLE REGULATORS AT THE STAGE OF LIVER METASTASIS. POLISH JOURNAL OF PATHOLOGY 65:100-112(2014).
Liedberg, F; Anderson, H; et al. Tissue microarray based analysis of prognostic markers in invasive bladder cancer: Much effort to no avail. UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS 26:17-24(2008).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.