Anti-FOXO3 polyclonal antibody (CPBT-66111RF)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Bovine, Chicken, Dog, Monkey, Mouse, Rat, Xenopus
Immunogen
Synthetic peptide corresponding to a portion of the amino acid sequence between 600-673 of
Conjugate
Unconjugated

Applications


Application Notes
Immunohistology - Paraffin: Min Dilution 10ug/ml; Western Blotting: 2 - 4ug/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
FOXO3; forkhead box O3; FOXO2; AF6q21; FKHRL1; FOXO3A
Entrez Gene ID
UniProt ID
O43524

Product Background


Pathway
AKT phosphorylates targets in the nucleus; AMPK signaling pathway; Adaptive Immune System; BDNF signaling pathway; Chemokine signaling pathway; Class I PI3K signaling events; Class I PI3K signaling events mediated by Akt; Constitutive PI3K/AKT Signaling in Cancer;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


CLEC3B p.S106G Mutant in a Caucasian Population of Successful Neurological Aging

JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES

Authors: Kolicheski, Ana; Walton, Ronald L.; Soto-Beasley, Alexandra, I; Heckman, Michael G.; Uitti, Ryan J.; Parfitt, Francine; Graff-Radford, Michelle R.; Wszolek, Zbigniew K.; Graff-Radford, Neill R.; Ross, Owen A.

A number of efforts are underway to better understand the role of genetic variation in successful aging and longevity. However, to date, only two genes have been consistently associated with longevity in humans: APOE and FOXO3, with the APOE epsilon 2 allele also protective against dementia. Recently, using an exome-wide SNP array approach, a missense variant CLEC3B c.316G>A (rs13963 p.S106G) was reported to associate with longevity in two independent cohorts of Japanese and Chinese participants. Interestingly, CLEC3B p.S106G is more frequent in Caucasian populations. Herein, we examined the frequency of CLEC3B p.S106G in a Caucasian series of 1,483 neurologically healthy individuals with a specific subset >80 years of age. Although our findings do not support an association between CLEC3B p.S106G and aging without neurological disease (p = .89), we confirmed the association between the APOE epsilon 2 allele and better survival without neurological disease (p = .001). Further assessment of healthy aged cohorts that retain intact neurological function will be critical to understand the etiology of neurodegenerative disease and the role of age at risk.

A circular RNA circ-DNMT1 enhances breast cancer progression by activating autophagy

ONCOGENE

Authors: Du, William W.; Yang, Weining; Li, Xiangmin; Awan, Faryal Mehwish; Yang, Zhenguo; Fang, Ling; Lyu, Juanjuan; Li, Feiya; Peng, Chun; Krylov, Sergey N.; Xie, Yizhen; Zhang, Yaou; He, Chengyan; Wu, Nan; Zhang, Chao; Sdiri, Mouna; Dong, Jun; Ma, Jian; Gao, Chunqi; Hibberd, Steven; Yang, Burton B.

Circular RNAs are a large group of noncoding RNAs that are widely expressed in mammalian cells. Genome-wide analyses have revealed abundant and evolutionarily conserved circular RNAs across species, which suggest specific physiological roles of these species. Using a microarray approach, we detected increased expression of a circular RNA circ-Dnmt1 in eight breast cancer cell lines and in patients with breast carcinoma. Silencing circ-Dnmt1 inhibited cell proliferation and survival. Ectopic circ-Dnmt1 increased the proliferative and survival capacities of breast cancer cells by stimulating cellular autophagy. We found that circ-Dnmt1-mediated autophagy was essential in inhibiting cellular senescence and increasing tumor xenograft growth. We further found that ectopically expressed circ-Dnmt1 could interact with both p53 and AUF1, promoting the nuclear translocation of both proteins. Nuclear translocation of p53 induced cellular autophagy while AUF1 nuclear translocation reduced Dnmt1 mRNA instability, resulting in increased Dnmt1 translation. From here, functional Dnmt1 could then translocate into the nucleus, inhibiting p53 transcription. Computational algorithms revealed that both p53 and AUF1 could bind to different regions of circ-Dnmt1 RNA. Our results showed that the highly expressed circular RNA circ-Dnmt1 could bind to and regulate oncogenic proteins in breast cancer cells. Thus circ-Dnmt1 appears to be an oncogenic circular RNA with potential for further preclinical research.

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