Anti-Human FOXO3 monoclonal antibody (CAB-5583RH)
Rabbit Anti-Human FOXO3 monoclonal antibody for WB, ICC/IF
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Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of FOXO3A on different lysates using anti- FOXO3A antibody at 1/1,000 dilution.
Lane 1: NIH/3T3;
Lane 2: PC-12
Lane 3: MCF-7
Fig2: ICC staining FOXO3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining FOXO3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining FOXO3A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1
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Ramamoorthy, S; Stepan, V; et al. Intracellular mechanisms mediating the anti-apoptotic action of gastrin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 323:44-48(2004).
Tao, RY; Xiong, XW; et al. FoxO3 Transcription Factor and Sirt6 Deacetylase Regulate Low Density Lipoprotein (LDL)-cholesterol Homeostasis via Control of the Proprotein Convertase Subtilisin/Kexin Type 9 (Pcsk9) Gene Expression. JOURNAL OF BIOLOGICAL CHEMISTRY 288:29252-29259(2013).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.