Anti-FOXO3 polyclonal antibody (DPABY-150)

Rabbit anti-Human FOXO3 (N-terminal) polyclonal antibody for IHC-Fr, ELISA, WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to 18 amino acid sequence from near the amino terminus of human FOXO3A
Conjugate
Unconjugated

Applications


Application Notes
Immunohistology - Frozen: 4ug/ml ; Western Blot: 0.5-1ug/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
FOXO3; forkhead box O3; FOXO2; AF6q21; FKHRL1; FOXO3A
Entrez Gene ID
UniProt ID

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Mitoxantrone induces apoptosis in osteosarcoma cells through regulation of the Akt/FOXO3 pathway

ONCOLOGY LETTERS

Authors: Park, See-Hyoung; Lee, Jongsung; Kang, Mi-Ae; Jang, Kyu Yun; Kim, Jung Ryul

The outcome of chemotherapy for osteosarcoma have improved during the past decade and more patients have access to combination chemotherapy, but there has been no significant clinical progress in the patient survival rate. Recently, forkhead-box O3 (FOXO3) was identified as a pivotal transcription factor responsible for the transcriptional regulation of genes associated with suppression of cancer. The purpose of the present study was to screen small chemicals activating FOXO3 and elucidate their underlying mechanism. Using a drug discovery platform based on the phosphorylation status of FOXO3 in osteosarcoma cells, mitoxantrone (MTZ), a type of DNA-damaging agent, was selected as a possible FOXO3 activator from the food and drug administration-approved drug library. MTZ treatments significantly inhibited the phosphorylation level of Akt-pS473 and caused nuclear localization of FOXO3 in osteosarcoma cells. MTZ treatment inhibited proliferation in osteosarcoma cells in vitro, whereas silencing FOXO3 potently attenuates MTZ-mediated apoptosis in osteosarcoma cells. Taken together, the results indicated that MTZ induces apoptosis in osteosarcoma cells through an Akt/FOXO3-dependent mechanism.

Paraquat treatment modulates integrin associated protein (CD47) and basigin (CD147) expression and mitochondrial potential on erythroid cells in mice

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

Authors: Bhardwaj, Nitin; Singh, Ashutosh

The present study is focused on the interaction of paraquat with the erythroid system in bone marrow and spleen of mice. Administration of paraquat (10 mg/kg of body weight i.p. on alternate days in C57Bl/6 mice) induced the level of reactive oxygen species in bone marrow (BM) on 7, 14, and 21 day time points but it was unchanged in spleen erythroid cell. A marked induction of CD147 expression in BM and spleen erythroid cells was observed in the paraquat treated mice. Paraquat treatment also modulated the CD47 expression in erythroid cells and its expression level was significantly higher on day 14, 21 and 28 in bone marrow and on day 14 and 21 in spleen. The expression level of mitochondrial potential and antioxidant genes SOD1, SOD2, GPX1 and FOXO3 expression was significantly reduced in BM erythroid cells but a reverse response was seen in spleen. Taken together, this study demonstrates that paraquat treatment modulates ROS production, mitochondrial membrane potential, and oxidative stress markers gene expression in the erythroid systems of C57Bl/6 mice.

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