Rabbit Anti-CASP3 Polyclonal Antibody (DPABH-24873)
Rabbit Anti-Human CASP3 Polyclonal Antibody for WB, IHC, IF
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Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of Jurkat cells, using CASP3 antibody at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3; caspase 3, apoptosis-related cysteine peptidase; CPP32; SCA-1; CPP32B; caspase-3
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Mitra, S; Khaidakov, M; et al. Prior exposure to oxidized low-density lipoprotein limits apoptosis in subsequent generations of endothelial cells by altering promoter methylation. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 301:H506-H513(2011).
Mi, S; Lee, XH; et al. Death receptor 6 negatively regulates oligodendrocyte survival, maturation and myelination. NATURE MEDICINE 17:816-U180(2011).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.