Rabbit Anti-CASP3 Polyclonal Antibody (DPABH-24873)

Rabbit Anti-Human CASP3 Polyclonal Antibody for WB, IHC, IF Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
Conjugate
unconjugated

Applications


Application Notes
WB:    1:500 - 1:2000; IHC:    1:50 - 1:200; IF:    1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of extracts of Jurkat cells, using CASP3 antibody at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).

Target


Alternative Names
CASP3; caspase 3, apoptosis-related cysteine peptidase; CPP32; SCA-1; CPP32B; caspase-3
Entrez Gene ID
UniProt ID

Citations


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References


Mitra, S; Khaidakov, M; et al. Prior exposure to oxidized low-density lipoprotein limits apoptosis in subsequent generations of endothelial cells by altering promoter methylation. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 301:H506-H513(2011).
Mi, S; Lee, XH; et al. Death receptor 6 negatively regulates oligodendrocyte survival, maturation and myelination. NATURE MEDICINE 17:816-U180(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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