Caspase 3 (Phospho-Ser150) ELISA Kit (DEIA-XYA878)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
2 x 96T
Sample
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The Caspase 3 (Phospho-Ser150) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 3 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Caspase 3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 3 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL
3. Quenching Buffer: 24 mL
4. Blocking Buffer: 50 mL
5. 10x Wash Buffer: 50 mL
6. 100x Anti-Caspase 3 (Phospho-Ser150) Antibody (Rabbit Polyclonal): 60 μL, red
7. 100x Anti-Caspase 3 Antibody (Rabbit Polyclonal): 60 μL, purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL, green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL, glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL, glass
11. Primary Antibody Diluent: 12 mL
12. Ready-to-Use Substrate: 12 mL
13. Stop Solution: 12 mL
14. Crystal Violet Solution: 12 mL
15. SDS Solution: 24 mL
16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Expression and activation of Daphnia pulex Caspase-3 are involved in regulation of aging

GENE

Authors: Tong, Qiaoqiong; Zhang, Mengmeng; Cao, Xiao; Xu, Shanliang; Wang, Danli; Zhao, Yunlong

Death-mediating proteases such as Caspases have been implicated in aging. Remarkably, active Caspase-3 can trigger widespread damage and degeneration, playing a key role in causing cell death. In order to explore the relationship between Caspase-3 and aging in Daphnia pulex, we cloned and analyzed the full-length cDNA sequence of its Caspase-3 gene. Both mRNA expression and activity of D. pulex Caspase-3 increased with age. Moreover, different forms of Caspase-3 appeared with aging. The expression of casp3-L was higher and decreased with age, while that of casp3-S was weak and increased with age, consistent with the trend in Caspase-3 activity. Mhc mRNA expression declined over time and was negatively correlated with age and Caspase-3. In situ hybridization results showed that Caspase-3 mRNA was expressed in different growth and reproduction stages, and its expression levels in embryos and larva were lower than that in adult D. pulex. Western blot analysis revealed the presence of Caspase-3 in the form of zymogens with a molecular weight of 36 kDa. Overall, this study explored age-associated gene regulation to provide a basis for the molecular mechanism of D. pulex reproductive conversion.

Genome-wide copy number profiling in gallbladder carcinoma - A study from north India

META GENE

Authors: Sharma, Aarti; Kumar, Ashok; Kumari, Niraj; Krishnani, Narendra; Rastogi, Neeraj

Gallbladder cancer (GBC) is a malignancy with poor prognosis and needs early diagnostic tools and newer therapeutics. Molecular profiling data in GBC is limited due to lack of enough high-throughput screening regarding its genetic information. The present study is carried out to determine copy number variations (CNVs), in 890 major cancer related genes in GBC patients. Eighteen histopathologically confirmed GBC cases were recruited. Tumor area was micro-dissected off from formalin fixed paraffin embedded tissue blocks having > 80% of tumor cells. DNA extraction was done by standard protocols. Copy number profiling was done by "OncoScan (TM) FFPE Assay Kit" (Affymetrix, CA). Data analysis was done by SNPFASST2 algorithm using Nexus Express Oncoscan software 3.0 & 7.5 (Biodiscovery, Inc., CA USA). A substantial number of CNVs detected in GBC were recurrent. Recurrent gains were at chromosome 12q14.2, 12q14.3, 20p12.1 and recurrent losses were at 4q35.1. The common genes affected in CN loss were NPP6, IRF2, CASP3, PRIMPOL, MLF1IP, CENPU, ACSL1, SLED1 in chromosome 4q35.1, whereas genes involved in CN gain were HMGA2, RPSAP52 on 12q14.3, RASSF3 gene on 12q14.2 and MACROD2 on 20p12.1. In conclusion, significant CNVs were found in GBC patients. More studies with larger sample size are suggested to validate these findings.

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