Anti-Factor V monoclonal antibody, clone BCW-5105

mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES. Herein, we performed analyses using the adjudin (a non-hormonal male contraceptive drug under development) model, wherein adjudin was known to perturb apical and basal ES function when used at high dose. Through direct administration of adjudin to the testis, adjudin at doses that failed to perturb BTB integrity per se, overexpression of an rpS6 phosphomimetic (i.e., constitutively active) mutant (i.e., p-rpS6-MT) that modified BTB function considerably potentiated adjudin efficacy. This led to disorderly spatial expression of proteins necessary to maintain the proper cytoskeletal organization of F-actin and microtubules (MTs) across the seminiferous epithelium, leading to germ cell exfoliation and aspermatogenesis. These findings yielded important insights regarding the role of mTORC1/rpS6 signaling complex in regulating BTB homeostasis.

This experiment used molecular marker technology to analyse high sugar genes, clone the target bands and the Random amplified polymorphic DNA (RAPD) molecular markers were converted into Sequence characterized amplified regions (SCAR) molecular markers, and additionally 120 RAPD primers were used to analyse female 3228B (sweet sorghum), paternal 3201B (ordinary sorghum) and their hybrids F-5. The results showed that 95 of 120 pairs amplified products, 25 of them did not amplify products, so the amplification rate reached 79%. The recombination rate of S512 and high sugar genes was 7% in F5 populations. S512 amplified polymorphic bands were recovered, cloned and sequenced, its band length was 441bp. According to the sequence, we transferred the RAPD to SCAR molecular markers and designed SCAR primer. We got the same amplification results with the RAPD in specific amplification progress of sweet sorghum F5 populations, thus the RAPD transfered to SCAR successfully, we called this SCAR(S512-441).