Anti-Factor V monoclonal antibody, clone BIW-5147

This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca. After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography. Crude SPs together with V45 and (F1-F8) were examined for their carbohydrate, protein, and sulfate contents. In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR). Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro. In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose. F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding. Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed. The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.

Objectives: This study aimed to examine the clinical and laboratory features of the patients diagnosed with food allergy who applied to the pediatric allergy outpatient clinic. Methods: This study was performed between March 2016 and December 2017 as a cross-sectional observational study. The files of 90 patients with food allergy were evaluated retrospectively. Results: Ninety patients were included in the study. Sixty three (70%) of the cases were male and 27 (30%) were female. The median age of the patients was 12 months (range 3-156), and the age at onset of symptoms was 4 months (1-156). At the time of the diagnosis, the total number of eosinophils was 410/mm(3) (0-4600), and the total IgE value was 83.1 IU/ml (3.17-2500). When the cases were divided into two groups according to their gender, no significant difference was found between the groups regarding the median age, onset age of the symptoms, total IgE, eosinophil and specific IgE levels. Fifty (55.6%) cases had atopic dermatitis, 31 (34.4%) had urticaria, 6 (6.7%) had proctocolitis, 2 (2.2%) had angioedema and 1 (1.1%) had anaphylaxis. Thirty-four (37.8%) of the cases had IgE-mediated, six (6.7%) cases had non-IgE mediated, and 50 (55.5%) cases had mixed type food allergy. The most common food allergens were egg 29 (32.2%), cow's milk and egg 27 (30%) and cow's milk 22 (24.4%). In the skin prick test, sensitivity was found in 52 (57.7%) patients. The most common sensitization was against egg (22.2%). Specific IgE values were found as F1: 0.87 kU/L (0.10-100), F2: 0.30 kU/L (0.10-96.90) and F5: 0.48 kU/L (0.10-53). Conclusion: Egg and cow's milk allergy were the most common food allergens in our study. However; more than half of the patients were diagnosed with atopic dermatitis. Evaluation of the patients with atopic dermatitis in terms of food allergy may be appropriate.