Anti-BUB3 monoclonal antibody, clone FQS6420(3)

Antibody Isotype

IgG

Clone

FQS6420(3)

Species Reactivity

Mouse; Rat; Human

Immunogen

A synthetic peptide corresponding to residues near the N terminus of Human Bub3 (UniProt ID: O43684)

Conjugate

Application Notes

WB: 1/10000 - 1/50000; IP: 1/10 - 1/100; IHC-P: 1/100 - 1/250; Flow Cyt: 1/10 - 1/100; ICC/IF: 1/250 - 1/500;

Alternative Names

BUB3; budding uninhibited; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; mitotic checkpoint protein BUB3; BUB3L; mitotic checkpoint component; budding uninhibited by benomyl; BUB3 budding uninhibited by benzimidazoles 3 homolog; hBUB3;

Entrez Gene ID

UniProt ID

Antigen Description

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Pathway

APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem;