Anti-BMI1 monoclonal antibody, clone 2.U.32 Knockout Validated

Antibody Isotype

IgG1

Clone

2.U.32

Species Reactivity

Mouse; Rat; Rabbit; Cat; Human; Xenopus laevis; Zebrafish

Immunogen

Recombinant fragment, corresponding to amino acids 1-202 of Mouse Bmi1

Conjugate

Application Notes

ICC/IF: 0.65 μg/ml; Flow Cyt: 0.65 μg/ml; WB: 0.2 - 2 μg/ml; IHC-P: 1/100.

Alternative Names

BMI1; BMI1 proto-oncogene, polycomb ring finger; PCGF4; RNF51; FLVI2/BMI1; polycomb complex protein BMI-1; flvi-2/bmi-1; ring finger protein 51; polycomb group protein Bmi1; BMI1 polycomb ring finger oncogene

Entrez Gene ID

UniProt ID

Antigen Description

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Pathway

Senescence and Autophagy, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;