Human ALB ELISA Matched Antibody Pair

Pollutants in the environment and exposure to them lead to medical ailments around the world. The present study aimed to evaluate the protective effect of evening primrose oil and fish oil against fenitrothion pesticide-induced toxicity in rats. Male Wistar rats weighing 150-220 g, were randomly distributed into seven groups, the first served as control. The second group received fenitrothion, the third and fourth groups were supplemented with fish oil and evening primrose oil, respectively. Rats of group five were exposed to fish oil and fenitrothion. The sixth group was exposed to evening primrose oil and fenitrothion. Rats of the seventh group were exposed to fish oil and evening primrose oil and fenitrothion. The results showed that the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and the levels of bilirubin (BIL), total protein (TP), albumin (ALB), glucose (GLU), cholesterol (CHOL), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), very low-density lipoproteins cholesterol (VLDL-c), and superoxide dismutase (SOD) were noticeably increased in rats administered fenitrothion. However, the level of serum high-density lipoprotein cholesterol (HDL-c) and glutathione (GSH) were markedly decreased. It was found that fish oil and evening primrose oil decreased the physiological unsettling influences initiated by fenitrothion. Moreover, the antioxidant properties of these oils supported the bioactive roles of its defensive impacts on fenitrothion toxicity. Finally, the present findings suggest that these oils may be utilized as preventive components against the toxicity of fenitrothion because of their antioxidant properties.

Background & Aim Shortage of donor hepatocytes limits hepatocyte transplantation for clinical application. Induced hepatic stem cells (iHepSCs) have capacities of self-renewal and bipotential differentiations. Here, we investigated whether iHepSCs could be extensively expanded, and whether they could differentiate into sufficient functional hepatocytes as donors for transplantation therapy after their extensive expansions. Methods Murine extensively expanded iHepSCs (50-55 passages) were induced to differentiate into iHepSC-Heps under a chemically defined condition. iHepSC-Heps were proved for carrying morphological hepatocyte characters and hepatocytic functions including low-density lipoprotein uptake, glycogen storage, CLF secretion, ICG uptake and release, Alb secretion, urea synthesis and metabolism-relative gene expressions respectively. Next, both iHepSCs and iHepSC-Heps were transplanted into Fah-/- mice respectively. Both liver repopulation and alleviation of liver function were compared between two transplantation groups. Results Murine iHepSCs still maintained the capacities of self-renewal and bipotential differentiations after extensive expansion. The efficiency for the functional hepatocyte differentiation from extensively expanded iHepSCs reached to 72.64%. Transplantations of both extensively expanded iHepSCs and iHepSC-Heps resulted in liver engraftment in Fah-/- mice. Survival rate of Fah-/- mice recipients and level of liver repopulation were 50% and 20.32 +/- 4.58% respectively in iHepSC-Heps group, while 33% and 10.4 +/- 4.3% in iHepSCs group. Conclusions Extensively expanded iHepSCs can efficiently differentiate into hepatocytes in chemical defined medium. Transplantation of iHepSC-Heps was more effective and more efficient than transplantation of iHepSCs in Fah-/- mice. Our results suggested an innovative system to obtain sufficient hepatocytes through hepatic differentiation of iHepSCs generated by lineage reprogramming.