A-RAF ELISA Kit

Name: A-RAF ELISA Kit
SKU: DEIA-XYA224
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse; Rat

Detection Method

Indirect ELISA

Intended Use

The A-RAF Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor A-RAF protein expression profile in cells. The kit can be used for measuring the relative amounts of A-RAF in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on A-RAF.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-A-RAF Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Oncogenic mutations in extramammary Paget's disease and their clinical relevance

INTERNATIONAL JOURNAL OF CANCER

Authors: Kang, Zhihua; Xu, Feng; Zhang, Qiao-an; Wu, Zhiyuan; Zhang, Xinju; Xu, Jinhua; Luo, Yan; Guan, Ming

Abstract

Extramammary Paget's disease (EMPD) is a rare cutaneous malignant neoplasm. The genetic alterations underlying its pathogenesis have less been described. Therefore, we analyzed the possible mutations in the KRAS, HRAS, NRAS, BRAF, ARAF, RAF1, PIK3CA, AKT1, CTNNB1 and APC genes as well as methylation and expression of CDH1 in 144 EMPD cases and 42 matched normal skin tissues. A distinct mutation profile was identified in EMPDs with 27 (19%) cases mutant for RAS and RAF genes and 50 (35%) cases harboring oncogenic mutations in PIK3CA and AKT1. Moreover, a mutually exclusive pattern was observed in the genetic variants in these two signaling pathways. No mutation was detected in CTNNB1 and APC genes. High prevalence of low expression and hypermethylation of CDH1 gene was detected in 33 and 48% of the EMPD cases, respectively. Furthermore, PIK3CA and AKT1 mutations were significantly correlated with CDH1 hypermethylation which could explain why the majority of EMPD cases with mutant PIK3CA and AKT1 were invasive. Our study demonstrates that genetic variants associated with constitutive activation of RAS/RAF and PI3K/AKT pathways are involved in the pathogenesis of EMPD. This may represent novel therapeutic targets for this skin cancer.

Structural characteristics of oxalate-soluble polysaccharides of Sosnowsky's hogweed (Heracleum sosnowskyi Manden)

CARBOHYDRATE POLYMERS

Authors: Makarova, Elena N.; Shakhmatov, Evgeny G.; Belyy, Vladimir A.

Abstract

Arabinogalactan proteins (AGP) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. The structural study has shown that a linear region of the pectic macromolecules consists of 1,4-alpha-D-galactopyranosyluronan blocks partially methyl esterified and acetylated. The branched region consists of 3-0- and partially 2-0-acetylated rhamnogalacturonan I. Side chains of the RG-I backbone include the regions of arabinogalactan I and branched 1,5-alpha-arabinan. The carbohydrate part of AGP consists of arabinogalactan II with a 1,3-beta-D-Galp main chain. The side chains of the branched area of AG-II are composed of 1,6-beta-D-Galp, 1,5-, 1,3,5-alpha-L-Araf, 4-0-Me-beta-D-GlcA and 1,4-beta-D-GlcpA, and non-reducing ends residues of beta-D-Galp, alpha-L-Araf, alpha-L-Rhap and alpha-L-Fucp. The branch points of the main and side chains are formed by 3,6-di-O-substituted beta-D-Galp. It was found that at least a portion of pectin is probably covalently linked to AGP, wherein AGP is linked to RG-I, but not with galacturonan. (C) 2016 Elsevier Ltd. All rights reserved.