Magic™ Anti-Human A RAF (Phospho S299) polyclonal antibody

A water-soluble pectic polysaccharide (MP-A40) was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru for the first time, with a molecular weight of 32,600 Da. MP-A40 was comprised of 68.63% galacturonic acid and 13.05% neutral sugar. In addition, arabinose, galactose, rhamnose, mannose and glucose composed the neutral sugar in a relative ratio of 4.94, 3.07, 2.13, 1.62 and 1.29% of the dry weight of MP-A40, respectively. Structural characterization of MP-A40 was investigated by methylation analysis and 1D/2D NMR spectroscopy. From the results, the structure of MP-A40 was revealed as follows: 1,4-linked alpha-D-GalpA and 1,4-linked alpha-D-GalpA6Me interspersed with rare t-Araf (0.60%), t-Rhap (1.67%) and t-GalpA (10.15%). Esterification assay showed that about 32% of the carboxylic groups in GalA residues existed as methyl ester. In addition, MP-A40 could inhibit the growth of human leukemic cell line K562 and stimulate nitric oxide production from RAW 264.7 macrophages both in dose-dependent manners. (C) 2014 Elsevier Ltd. All rights reserved.

Black soybeans (Glycine max (L.) Merr.) have been widely used as a health food and medicinal herb in oriental medicine. In the present study, the chemical structures of two water-soluble polysaccharides (black soybean polysaccharide 1 (BSPS-1) and black soybean polysaccharide 3 (BSPS-3)) isolated from black soybeans were characterized by high performance size-exclusion chromatography (HPSEC), methylation analysis, and 1D (H-1, C-13) and 2D (COSY, TOCSY, HSQC, NOESY, and HMBC) NMR spectra. The molecular weights of BSPS-1 and BSPS-3 were 1.95 x 10 (5)and 1.88 x 10 (5) Da, respectively. Methylation analysis and NMR spectra indicate that BSPS-1 is composed of 1,6-alpha-d-glucopyranosyl residues. By contrast, BSPS-3 is mainly composed of a 1,3-beta-d-galactopyranosyl residue backbone with side chains substituted at the O-6 position consisting of large content of T-alpha-(L)-Araf-(1 -> residues, and small contents of -> 5)-alpha-l-Araf-(1 ->, -> 2)-a-l-Rhap-(1 ->, and 4-O-Me-beta-d-GlcAp-(1 -> residues. Our results suggest that BSPS-1 is a linear (1 -> 6)-a-d-glucan, whereas BSPS-3 is a type II arabinogalactan. The unique structures of BSPS-1 and BSPS-3 indicate that they might have wide applications in food and pharmaceutical industries.