A-RAF (Phospho-Tyr302) ELISA Kit

Name: A-RAF (Phospho-Tyr302) ELISA Kit
SKU: DEIA-XYA225
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse; Rat

Detection Method

Indirect ELISA

Intended Use

The A-RAF (Phospho-Tyr302) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor A-RAF protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated A-RAF in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on A-RAF phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 10x Wash Buffer: 50 mL (10x)
6. 100x Anti-A-RAF (Phospho-Tyr302) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-A-RAF Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 4 seals

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

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Q: I would like to determine the amount of dexamethason that is encapsulated in amphiphilic nanoparticles. I wonder if you could tell me that my system is more similar to milk or blood samples. Could you please advise?

A: Do you know if the dexamethasone is encapsulated inside the amphiphilic? If the dexamethasone is encapsulated inside the amphiphilic (like in a liposome), then you won't be able to detect it with ELISA. You'll have to break open the nanoparticles prior to assaying for dexamethasone.

Customer Reviews

References

Characterization of Structures and Antiviral Effects of Polysaccharides from Portulaca oleracea L.

CHEMICAL & PHARMACEUTICAL BULLETIN

Authors: Dong, Cai-Xia; Hayashi, Kyoko; Lee, Jung-Bum; Hayashi, Toshimitsu

Abstract

Three polysaccharides including a neutral polysaccharide (RN), an acidic polysaccharide (RA) and a pectic polysaccharide (RP) were isolated from aerial part of Portalaca oleracea L. and evaluated for their anti-herpes simplex virus type 2 (HSV-2) and anti-influenza A virus (IFV-A). RN was found to consist of glucose (Glc), mannose (Man) and arabinose (Ara) with small amounts of galactose (Gal), and identified to be an arabinoglucomannan. RA was mainly composed of Gal and Ara with a small proportion of glucuronic acid (GlcA). It was characterized as a type II arabinogalactan (AGII), which consisted of a 1,3-, 1,6- and 1,3,6-linked galactopyranosyl (Galp) and non-reducing terminal and 1,5-linked arabinofuranosyl (Araf) residues. RP was deduced to be a pectin, which consisted of a predominant amount of galacturonic acid (GalA) with small amounts of Gal, rhamnose (Rha) and Ara. The GalA residues were found to be highly methyl-esterified and partially acetylated. Results of antiviral tests showed that only RP had anti-HSV-2 activity. Furthermore, its anti-HSV-2 target was elucidated to be the step of virus penetration into host cells. No marked virucidal activity of RP was observed.

Longan pulp polysaccharides relieve intestinal injury in vivo and in vitro by promoting tight junction expression

CARBOHYDRATE POLYMERS

Authors: Bai, Yajuan; Huang, Fei; Zhang, Ruifen; Dong, Lihong; Jia, Xuchao; Liu, Lei; Yi, Yang; Zhang, Mingwei

Abstract

The integrity of the intestinal mucosal barrier is important for the health of the host. In this study, longan pulp polysaccharides (LP) prevented the intestinal mucosal injury by increasing the expression of mucin 2, tight junction proteins zonulae occludens-1 (ZO-1), claudin-1, claudin-4, and adherens junction E-cadherin in cyclophosphamide-treated mice. To further identify the principle bioactive component of LP, four acidic polysaccharides (LPIa, LPIIa, LPIIIa, and LPIVa) were purified, and their intestinal protection activity in vitro was compared. LPIa, LPIIa, and LPIIIa displayed an ability to increase mRNA expression of ZO-1, claudin-1, occludin, and E-cadherin in differentiated Caco-2 cells, especially LPIa. LPIa has specific structure characteristics: porous surface structure, a high molecular weight (1.47 x 105 Da), and two specific glycosidic linkages of a-Araf-(1 > and 5)-a-Araf-(1. These structure characteristics might primarily contribute to greater intestinal barrier protective effect of LPIa.