Rabbit anti-Arctic char vitellogenin Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Arctic char (Salvelinus alpinus); Atlantic salmon (Salmo salar); Brown trout (Salmo trutta); Atlantic cod (Gadus morhua); Turbot (Scophthalmus maximus); flounder (Platichthys flesus); wrasse (Ctenolabrus rupestris); tilapia (Oreochromis niloticus); Zebrafish (Danio rerio)

Immunogen

Vitellogenin purified from plasma of 17β-estradiol treated Arctic char (Salvelinus alpinus).

Conjugate

Application Notes

ELISA: 1:500 - 1:3000; Western blot: 1:500 - 1:3000
The polyclonal antibody PO-1 works well in both ELISA and western blot for a number of species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.

General Notes

This product consists of rabbit polyclonal antibodies (affinity-purified IgG fraction) against vitellogenin from Arctic char (Salvelinus alpinus).
The polyclonal antibody binds to vitellogenin in plasma samples from Arctic char (Salvelinus alpinus). The antibody also cross-reacts with vitellogenin from Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus), flounder (Platichthys flesus), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus) and zebrafish (Danio rerio). The degree of cross-reactivity differs between species and with the methods employed.
The polyclonal antibody works well in both ELISA and western blot for a number of species.