Mouse anti-salmon vitellogenin Monoclonal antibody, clone CO-6

Antibody Isotype

IgG1, κ

Clone

CO-6

Species Reactivity

Atlantic salmon (Salmo salar); Brown trout (Salmo trutta); Rainbow trout (Oncorhynchus mykiss); Arctic char (Salvelinus alpinus); wrasse (Ctenolabrus rupestris)

Immunogen

Vitellogenin purified from plasma of 17β-estradiol treated Atlantic salmon (Salmo salar).

Conjugate

Application Notes

ELISA: 1:100 - 1:2000;
The monoclonal antibody BN-5 works well in ELISA for a number of species. The antibody may also work in western blot for some fish species, but the affinity for vitellogenin is much lower when this method is used. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.

General Notes

This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from Atlantic salmon (Salmo salar).
The monoclonal antibody binds with high affinity to vitellogenin in plasma samples from Atlantic salmon (Salmo salar). The antibody cross-reacts with vitellogenin in plasma samples from brown trout (Salmo trutta), rainbow trout (Oncorhynchus mykiss), Arctic char (Salvelinus alpinus) and wrasse (Ctenolabrus rupestris). The degree of cross-reactivity differs between species and with the methods employed.
The monoclonal antibody works well in ELISA for a number of species. The antibody may also work in western blot for some fish species, but the affinity for vitellogenin is much lower when this method is used.