Vitellogenin purified from plasma of 17β-estradiol treated striped bass (Morone saxatilis).
Conjugate
Unconjugated
Applications
Application Notes
ELISA: 1:100 - 1:1000; Western blot: 1:500 - 1:1000 The monoclonal antibody ND-1C8 works well in both ELISA and western blot for a number of species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.
General Notes
This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from striped bass (Morone saxatilis). The monoclonal antibody binds with high affinity to vitellogenin from striped bass (Morone saxatilis) and rainbow trout (Oncorhynchus mykiss). The antibody also cross-reacts with vitellogenin from a variety of different species including largemouth bass (Microptenes salmonides), Atlantic cod (Gadus morhua), tilapia (Oreochromis niloticus), killifish (Fundulus heteroclitus) and sheepshead minnow (Cyprinodon variegatus). The degree of cross-reactivity differs between species and with the methods employed. The monoclonal antibody works well in both ELISA and western blot for a number of species.
Citations
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