Specifications
Species Reactivity
Brown bullhead (Ameiurus nebulosus)
Immunogen
Vitellogenin purified from brown bullhead (Ameirus nebulosus), sheephead minnow (Cyprinodon variegatus), bowfin (Amia calva) and spotted gar (Lepisosteus oculatus).
Applications
Application Notes
ELISA: 1:100 - 1:1000; Western blot: 1:100 - 1:500
The monoclonal antibody ND-1D12 works well in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.
General Notes
This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from brown bullhead (Ameiurus nebulosus).
The monoclonal antibody binds with high affinity to vitellogenin from brown bullhead (Ameiurus nebulosus). The antibody does not cross-react with vitellogenin from sheepshead minnow (Cyprinodon variegatus), bowfin (Amia calva), spotted gar (Lepisosteus oculatus), killifish (Fundulus heteroclitus), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus), rainbow trout (Oncorhynchus mykiss), carp (Cyprino carpio) or zebrafish (Danio rerio). The degree of cross-reactivity may differ between species and with the methods employed.
The monoclonal antibody works well in both ELISA and western blot.
Citations
Publication ()
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COA
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