Mouse anti-killifish vitellogenin Monoclonal antibody, clone OE-6G9

Antibody Isotype

IgG1, κ

Clone

OE-6G9

Species Reactivity

killifish (Fundulus heteroclitus); white perch (Morone americanus); common carp (Cyprinus carpio); wrasse (Ctenolabrus rupestris); tilapia (Oreochromis niloticus); halibut (Hippoglossus hippoglossus); Sheepshead minnow (Cyprinodon variegatus)

Immunogen

Vitellogenin purified from plasma of 17β-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus) and bowfin (Amia calva). Egg yolk from swordfish (Xiphias gladius).

Conjugate

Application Notes

ELISA: 1:100 - 1:1000; Western blot: 1:100 - 1:500
The monoclonal antibody ND-5F8 works well in both ELISA and western blot for a number of species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.

General Notes

This product consists of affinity-purified mouse monoclonal antibodies against vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus).
The monoclonal antibody binds with high affinity to vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). The antibody also cross-reacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). The antibody does not cross-react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius). The degree of cross-reactivity differs between species and with the methods employed.
The monoclonal antibody works well in both ELISA and western blot for a number of species.