THC ELISA Kit

Sample

Urine, Blood, Oral Fluid, Breast Milk

Species Reactivity

Human, Research Animal

Detection Method

cELISA

Intended Use

For the determination of trace quantities of THC metabolite and Parent THC in human urine, blood, or oral fluid.

Contents of Kit

1. EIA Buffer: 30 mL (ready-to-use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of samples.
2. Wash Buffer Concentrate (10×): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. Substrate: 20 mL (ready-to-use). Stabilized 3, 3', 5, 5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light sensitive.
4. Drug-Enzyme Conjugate: 14 mL (ready-to-use). Drug-horseradish peroxidase conjugate. Do not dilute.
5. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
6. Acid Stop Solution: 14 mL (ready-to-use). 1 N H₂SO₄ used to stop the enzyme reaction.
7. Whole Blood Cut-off Calibrators: DO NOT DILUTE.
a. Blood Negative Calibrator: 1 mL provided in a synthetic matrix (red cap).
b. Blood Cut-off Calibrator-(THC-COOH): 1 mL provided in a synthetic matrix, 5 ng/mL (red cap).
8. Urine Cut-off Calibrators: DO NOT DILUTE.
a. Urine Negative Calibrator: 1 mL provided in a synthetic matrix (yellow cap).
b. Urine Cut-off Calibrator-(THC-COOH): 1 mL provided in a synthetic matrix, 50 ng/mL (yellow cap).

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C.

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

Creative Diagnostics's THC ELISA Kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for research use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Standard Curve