Product Overview
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP9 Protein (YP_009725305.1) (His1-Lys113) was expressed with a polyhistidine tag at the N-terminusand and a C-terminal AVI tag.
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) NSP9 Protein consists of 140 amino acids and predicts a molecular mass of 15.46 kDa.
Alternative Names
SARS-CoV-2 NSP9; SARS-CoV-2
Purity
> 90 % as determined by SDS-PAGE.
Buffer
Lyophilized from sterile 50mM Tris, 150mM NaCl, 2mM βME, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) is comprised of a large single stranded positive polarity RNA genome
that acts as messenger RNA after entering the host. Post infection, the ssRNA encodes two open reading frames, produced through host ribosomal frameshifting that transcribes two polyprotein products. The polyprotein products are subsequently cleaved into 27 viral proteins by internally encoded proteases. Further processing of the polyprotein releases an RNA-polymerase along with several non-structural proteins that facilitate RNA synthesis and may play a role in the enveloping process but are not included in the viral coat.
Keywords
SARS-CoV-2 NSP9; SARS-CoV-2
Citations
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