Product Overview
Recombinant Severe acute respiratory syndrome coronavirus 2 Non-structural protein 9(nsp9), C-terminal hFC-Flag-tagged.
Molecular Weight
44.5 kDa
Alternative Names
SARS-CoV-2 NSP9; SARS-CoV-2
Purity
Greater than 90% as determined by SDS-PAGE.
Buffer
The buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage
Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Introduction
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) is comprised of a large single stranded positive polarity RNA genome
that acts as messenger RNA after entering the host. Post infection, the ssRNA encodes two open reading frames, produced through host ribosomal frameshifting that transcribes two polyprotein products. The polyprotein products are subsequently cleaved into 27 viral proteins by internally encoded proteases. Further processing of the polyprotein releases an RNA-polymerase along with several non-structural proteins that facilitate RNA synthesis and may play a role in the enveloping process but are not included in the viral coat.
Keywords
SARS-CoV-2 NSP9; SARS-CoV-2
Citations
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