Recombinant SARS-CoV-2 NSP3 [His] (DAGC200)

Recombinant SARS-CoV-2 NSP3 from E. coli [His]

Product Overview
Recombinant SARS-CoV-2 NSP3 is produced by E.coli expression system and the target gene encoding Glu1024-Gln1198 is expressed with N-His Tag.
Target
SARS-CoV-2 NSP3
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
Predicted molecular weight 22.03kDa
Alternative Names
SARS-CoV-2 NSP3; SARS-CoV-2
Procedure
None
Purity
> 90 % as determined by SDS-PAGE.
Format
Liquid/Lyophilized
Size
50ug; 100ug; 1mg
Buffer
Supplied as solution form in PBS, pH 7.5/Supplied as lyophilized from PBS,pH 7.5
Preservative
None
Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Introduction
The multi-domain non-structural protein 3 (Nsp3) is the largest protein encoded by the coronavirus (CoV) genome, with an average molecular mass of about 200 kD. Nsp3 is an essential component of the replication/transcription complex. It comprises various domains, the organization of which differs between CoV genera, due to duplication or absence of some domains. However, eight domains of Nsp3 exist in all known CoVs: the ubiquitin-like domain 1 (Ubl1), the Glu-rich acidic domain (also called "hypervariable region"), a macrodomain (also named "X domain"), the ubiquitin-like domain 2 (Ubl2), the papain-like protease 2 (PL2pro), the Nsp3 ectodomain (3Ecto, also called "zinc-finger domain"), as well as the domains Y1 and CoV-Y of unknown functions. In addition, the two transmembrane regions, TM1 and TM2, exist in all CoVs.
Keywords
SARS-CoV-2 NSP3; SARS-CoV-2

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