Product Overview
Recombinant SARS-CoV-2 NSP15 is produced by E.coli expression system and the target gene encoding Gln6452-Gln6798 is expressed with N-His Tag.
Molecular Weight
Predicted molecular weight 41.24kDa
Alternative Names
SARS-CoV-2 NSP15; SARS-CoV-2
Purity
> 90 % as determined by SDS-PAGE.
Format
Liquid/Lyophilized
Buffer
Supplied as solution form in PBS, pH 7.5/Supplied as lyophilized from PBS,pH 7.5
Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Introduction
Nonstructural protein 15 (nsp15) encodes an endoribonuclease that is highly conserved among vertebrate nidoviruses (coronaviruses and arteriviruses) and plays a critical role in viral replication and transcription. Nsp15 is an IFN antagonist and it inhibits interferon-β production via an endoribonuclease activity-independent mechanism. Importantly, Nsp8 was found to interact with both monomeric and hexameric Nsp15. The Nsp7/Nsp8 complex displays a higher binding affinity for Nsp15. Furthermore, Nsp8 and the Nsp7/Nsp8 complex also enhance the NendoU activity of hexameric Nsp15 in vitro Taking the findings together.
Keywords
SARS-CoV-2 NSP15; SARS-CoV-2
Citations
Publication ()
Have you cited DAGC204 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
