Tag/Conjugate
Unconjugated
Molecular Weight
26.3 kDa
Alternative Names
Hepatitis C virus Non Structural Protein 4; HCV; HCV NS4; HCV NS-4
Purity
98%, analysed by SDS-PAGE (reducing and non-reducing) followed by Coomassie-Blue staining and immunoprobing of Western blotted proteins with anti-E.coli serum (DAKO)
Buffer
Antigen specific solution, contains either 8M Urea + 2mM DTE or 0.1% SDS.
Storage
At -80°C 10 years from the date of production. Avoid repeated freeze-thaw cycles
Introduction
HCV is a small 50nm, enveloped, single-stranded, positive sense RNA virus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host''s immune response. Hepatitis C virus is classified into six genotypes (1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).
Antigen Description
Recombinant Hepatitis C virus NS4 is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 252 amino acids with a molecular mass of 26300 Da. The product represents amino acids 1617 to 1864 of the HCV polyprotein, covering the C terminal end (45 a.a.) of NS3, the complete NS4A sequence (54 a.a.) and the major part of NS4B (153 a.a. of the N-terminus).
Keywords
Hepatitis C virus Non Structural Protein 4; HCV; HCV NS4; HCV NS-4
Citations
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