HCV Nonstructural Protein 4 [GST] (DAG536)

HCV Nonstructural Protein 4 [GST], recombinant protein from E. coli Datasheet

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Product Overview
Recombinant Hepatitis C Virus antigen (66 kDa), contains an artificial mosaic polypeptide composite constructed from fragments of the NS-4 immunodominant region of 11 different HCV genotypes and a GST fusion partner, was expressed in E. coli, and puried i
Nature
Recombinant
Tag/Conjugate
GST
Molecular Weight
66 kDa
Procedure
1mM EDTA
Purity
> 95% pure (10% PAGE coomassie staining). S-Sepharose and Ceramic Hydroxyapatite and Affinity purification
Format
Purified, Liquid
Concentration
1 mg/ml
Buffer
8M urea, 20mM TRIS, pH 9.0 containing 10mM DTT, 1mM EDTA and 0.5% Tween-20
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Antigen Description
NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
Keywords
HCV; HCV NS4; Hepatitis C Virus nonstructural antigen 4; Non structural protein 4A; Non structural protein 4B; NS4A; NS4B; Flaviviridae; Hepacivirus; Hepatitis C virus; p27; p8; Hepatitis C Virus NS4

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